Anti-Mouse CD25 PE

Also known as: Interleukin-2 Receptor alpha

Clone: PC61.5

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 12-0251

Cat. No. Size
12-0251-81 50 ug
12-0251-82 100 ug
12-0251-83 200 ug
Please inquire about custom configurations or bulk packaging.

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Data for Anti-Mouse CD25 PE.

Staining of C57Bl/6 splenocytes with Anti-Mouse CD4 FITC (cat. 11-0041) and 0.06 ug of Rat IgG1 K...View More

  • Data for Anti-Mouse CD25 PE.
Description

Description: The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

Details
Host Rat
Isotype IgG1, lambda
Reactivity Mouse
Conjugate PE
Laser Blue Laser, Green Laser, Yellow-Green Laser
Emit 575 nm
Excite 488 - 561 nm
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
14-0251 Anti-Mouse CD25 Purified FC RUO
48-0251 Anti-Mouse CD25 eFluor® 450 405 nm 450 nm FC RUO
13-0251 Anti-Mouse CD25 Biotin FC RUO
15-0251 Anti-Mouse CD25 PE-Cyanine5 488 - 561 nm FC RUO
45-0251 Anti-Mouse CD25 PerCP-Cyanine5.5 488 nm FC RUO
47-0251 Anti-Mouse CD25 APC-eFluor® 780 633 - 647 nm 780 nm FC RUO
35-0251 Anti-Mouse CD25 PE-Cyanine5.5 488 - 567 nm 690 nm FC RUO
36-0251 Anti-Mouse CD25 Functional Grade Biotin FC RUO
56-0251 Anti-Mouse CD25 Alexa Fluor® 700 633 - 647 nm 723 nm FC RUO
25-0251 Anti-Mouse CD25 PE-Cyanine7 488 - 561 nm 775 nm FC RUO
53-0251 Anti-Mouse CD25 Alexa Fluor® 488 488 nm 519 nm FC RUO
17-0251 Anti-Mouse CD25 APC 633 - 647 nm 660 nm FC RUO
16-0251 Anti-Mouse CD25 Functional Grade Purified FC, FA RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
11-0041 Anti-Mouse CD4 FITC 488 nm 518 nm FC RUO
12-4301 Rat IgG1 K Isotype Control PE 488 - 561 nm FC RUO
17-5773 Anti-Mouse/Rat Foxp3 APC 633 - 647 nm 660 nm FC RUO
References

References: Tuve S, Chen BM, Liu Y, Cheng TL, Touré P, Sow PS, Feng Q, Kiviat N, Strauss R, Ni S, Li ZY, Roffler SR, Lieber A. Combination of tumor site-located CTL-associated antigen-4 blockade and systemic regulatory T-cell depletion induces tumor-destructive immune responses. Cancer Res. 2007 Jun 15;67(12):5929-39. (PC61.5, in vivo depletion, PubMed)

Huang B, Zhao J, Shen S, Li H, He KL, Shen GX, Mayer L, Unkeless J, Li D, Yuan Y, Zhang GM, Xiong H, Feng ZH. Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling. Cancer Res. 2007 May 1;67(9):4346-52. (PC61.5, in vivo depletion, PubMed)

Hayashi T, Hasegawa K, Adachi C. Elimination of CD4+CD25+ T cell accelerates the development of glomerulonephritis during the preactive phase in autoimmune-prone female NZB × NZW F1 mice. Int J Exp Pathol. 2005 Oct;86(5):289-96. (PC61.5, in vivo depletion, PubMed)

Lowenthal JW, Corthésy P, Tougne C, Lees R, MacDonald HR, Nabholz M. High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61. J Immunol. 1985 Dec;135(6):3988-94.

Lowenthal JW, Zubler RH, Nabholz M, MacDonald HR. Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes. Nature. 1985 Jun 20-26;315(6021):669-72.

Lowenthal JW, Tougne C, MacDonald HR, Smith KA, Nabholz M. Antigenic stimulation regulates the expression of IL 2 receptors in a cytolytic T lymphocyte clone. J Immunol. 1985 Feb;134(2):931-9.