Anti-Mouse CD25 PE-Cyanine7

Also known as: Interleukin-2 Receptor alpha

Clone: PC61.5

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 25-0251

Cat. No. Size
25-0251-81 50 ug
25-0251-82 100 ug
Please inquire about custom configurations or bulk packaging.

To enable ordering, please select your country. Cookies will need to be enabled to continue on our website. View our Privacy Policy.

Data for Anti-Mouse CD25 PE-Cy7.

Staining of C57Bl/6 splenocytes with Anti-Mouse CD4 FITC (cat. 11-0041) and 0.06 ug of Rat IgG1 K...View More

  • Data for Anti-Mouse CD25 PE-Cy7.
Description

Description: The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

Details
Host Rat
Isotype IgG1, lambda
Reactivity Mouse
Conjugate PE-Cyanine7
Laser Blue Laser, Green Laser, Yellow-Green Laser
Emit 775 nm
Excite 488 - 561 nm
Legal FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT # 5,268,486, 5,569,587 AND 5,627,027 AND FOREIGN EQUIVALENTS AND PENDING APPLICATIONS. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE. COMMERCIAL USE shall include: 1. sale, lease, license or other transfer of the material or any material derived or produced from it; 2. sale, lease, license or other grant of rights to use this Material or any material derived or produced from it; 3. use of this material to perform services for a fee for third parties. IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO EBIOSCIENCE, INC. 10255 SCIENCE CENTER DRIVE, SAN DIEGO, CALIFORNIA 92121 USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
15-0251 Anti-Mouse CD25 PE-Cyanine5 488 - 561 nm FC RUO
16-0251 Anti-Mouse CD25 Functional Grade Purified FC, FA RUO
12-0251 Anti-Mouse CD25 PE 488 - 561 nm 575 nm FC RUO
17-0251 Anti-Mouse CD25 APC 633 - 647 nm 660 nm FC RUO
53-0251 Anti-Mouse CD25 Alexa Fluor® 488 488 nm 519 nm FC RUO
14-0251 Anti-Mouse CD25 Purified FC RUO
45-0251 Anti-Mouse CD25 PerCP-Cyanine5.5 488 nm FC RUO
48-0251 Anti-Mouse CD25 eFluor® 450 405 nm 450 nm FC RUO
13-0251 Anti-Mouse CD25 Biotin FC RUO
56-0251 Anti-Mouse CD25 Alexa Fluor® 700 633 - 647 nm 723 nm FC RUO
35-0251 Anti-Mouse CD25 PE-Cyanine5.5 488 - 567 nm 690 nm FC RUO
36-0251 Anti-Mouse CD25 Functional Grade Biotin FC RUO
47-0251 Anti-Mouse CD25 APC-eFluor® 780 633 - 647 nm 780 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
11-0041 Anti-Mouse CD4 FITC 488 nm 518 nm FC RUO
12-5773 Anti-Mouse/Rat Foxp3 PE 488 - 561 nm FC RUO
25-4301 Rat IgG1 K Isotype Control PE-Cyanine7 488 - 561 nm 775 nm FC RUO
References

References: Tuve S, Chen BM, Liu Y, Cheng TL, Touré P, Sow PS, Feng Q, Kiviat N, Strauss R, Ni S, Li ZY, Roffler SR, Lieber A. Combination of tumor site-located CTL-associated antigen-4 blockade and systemic regulatory T-cell depletion induces tumor-destructive immune responses. Cancer Res. 2007 Jun 15;67(12):5929-39. (PC61.5, in vivo depletion, PubMed)

Huang B, Zhao J, Shen S, Li H, He KL, Shen GX, Mayer L, Unkeless J, Li D, Yuan Y, Zhang GM, Xiong H, Feng ZH. Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling. Cancer Res. 2007 May 1;67(9):4346-52. (PC61.5, in vivo depletion, PubMed)

Hayashi T, Hasegawa K, Adachi C. Elimination of CD4+CD25+ T cell accelerates the development of glomerulonephritis during the preactive phase in autoimmune-prone female NZB × NZW F1 mice. Int J Exp Pathol. 2005 Oct;86(5):289-96. (PC61.5, in vivo depletion, PubMed)

Lowenthal JW, Corthésy P, Tougne C, Lees R, MacDonald HR, Nabholz M. High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61. J Immunol. 1985 Dec;135(6):3988-94.

Lowenthal JW, Zubler RH, Nabholz M, MacDonald HR. Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes. Nature. 1985 Jun 20-26;315(6021):669-72.

Lowenthal JW, Tougne C, MacDonald HR, Smith KA, Nabholz M. Antigenic stimulation regulates the expression of IL 2 receptors in a cytolytic T lymphocyte clone. J Immunol. 1985 Feb;134(2):931-9.