Anti-Mouse CD25 eFluor® 450

Description: The eBio3C7 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of the IL-2 receptor is inhibited by the 3C7 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) and subsequent detection with another CD25 antibody.

References: Klug DB, Carter C, Crouch E, Roop D, Conti CJ, Richie ER. Interdependence of cortical thymic epithelial cell differentiation and T-lineage commitment. Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11822-7. (eBio3C7, IH/F, PubMed)

Ortega G, Robb RJ, Shevach EM, Malek TR. The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells.J Immunol. 1984 Oct;133(4):1970-5.