Anti-Mouse CD25 Biotin

Description: The eBio7D4 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage, as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) followed by subsequent detection with another one. eBio7D4 has been reported to block IL-2 binding but can be overcome by additional IL-2. The monoclonal antibody PC61.5 (cat. 16-0251) is recommended for in vivo studies.

References: Malek TR, Robb RJ, Shevach EM.Identification and initial characterization of a rat monoclonal antibody reactive with the murine interleukin 2 receptor-ligand complex. Proc Natl Acad Sci U S A. 1983 Sep;80(18):5694-8.

Ortega G, Robb RJ, Shevach EM, Malek TR.The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells. J Immunol. 1984 Oct;133(4):1970-5.