Anti-Mouse CD25 Alexa Fluor® 488

Also known as: Interleukin-2 Receptor alpha

Clone: PC61.5

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-0251

Cat. No.	Size
53-0251-80	25 ug
53-0251-82	100 ug
Please inquire about custom configurations or bulk packaging.

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Staining of BALB/c splenocytes with Anti-Mouse CD4 PE (cat. 12-0042) and 0.06 ug of Rat IgG1 k...View More

Description

Description: The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

Details

Host	Rat
Isotype	IgG1, lambda
Reactivity	Mouse
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
45-0251	Anti-Mouse CD25 PerCP-Cyanine5.5	488 nm		FC	RUO
14-0251	Anti-Mouse CD25 Purified			FC	RUO
48-0251	Anti-Mouse CD25 eFluor® 450	405 nm	450 nm	FC	RUO
13-0251	Anti-Mouse CD25 Biotin			FC	RUO
25-0251	Anti-Mouse CD25 PE-Cyanine7	488 - 561 nm	775 nm	FC	RUO
16-0251	Anti-Mouse CD25 Functional Grade Purified			FC, FA	RUO
56-0251	Anti-Mouse CD25 Alexa Fluor® 700	633 - 647 nm	723 nm	FC	RUO
47-0251	Anti-Mouse CD25 APC-eFluor® 780	633 - 647 nm	780 nm	FC	RUO
36-0251	Anti-Mouse CD25 Functional Grade Biotin			FC	RUO
35-0251	Anti-Mouse CD25 PE-Cyanine5.5	488 - 567 nm	690 nm	FC	RUO
12-0251	Anti-Mouse CD25 PE	488 - 561 nm	575 nm	FC	RUO
15-0251	Anti-Mouse CD25 PE-Cyanine5	488 - 561 nm		FC	RUO
17-0251	Anti-Mouse CD25 APC	633 - 647 nm	660 nm	FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
12-0042	Anti-Mouse CD4 PE	488 - 561 nm	575 nm	FC	RUO
53-0252	Anti-Mouse CD25 Alexa Fluor® 488	488 nm	519 nm	FC	RUO
53-0253	Anti-Mouse CD25 Alexa Fluor® 488	488 nm	519 nm	FC	RUO
53-0259	Anti-Human CD25 Alexa Fluor® 488	488 nm	519 nm	FC	RUO
53-4301	Rat IgG1 K Isotype Control Alexa Fluor® 488	488 nm	519 nm	FC, ICC, IHC	RUO

References

References: Tuve S, Chen BM, Liu Y, Cheng TL, Touré P, Sow PS, Feng Q, Kiviat N, Strauss R, Ni S, Li ZY, Roffler SR, Lieber A. Combination of tumor site-located CTL-associated antigen-4 blockade and systemic regulatory T-cell depletion induces tumor-destructive immune responses. Cancer Res. 2007 Jun 15;67(12):5929-39. (PC61.5, in vivo depletion, PubMed)

Huang B, Zhao J, Shen S, Li H, He KL, Shen GX, Mayer L, Unkeless J, Li D, Yuan Y, Zhang GM, Xiong H, Feng ZH. Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling. Cancer Res. 2007 May 1;67(9):4346-52. (PC61.5, in vivo depletion, PubMed)

Hayashi T, Hasegawa K, Adachi C. Elimination of CD4+CD25+ T cell accelerates the development of glomerulonephritis during the preactive phase in autoimmune-prone female NZB × NZW F1 mice. Int J Exp Pathol. 2005 Oct;86(5):289-96. (PC61.5, in vivo depletion, PubMed)

Lowenthal JW, Corthésy P, Tougne C, Lees R, MacDonald HR, Nabholz M. High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61. J Immunol. 1985 Dec;135(6):3988-94.

Lowenthal JW, Zubler RH, Nabholz M, MacDonald HR. Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes. Nature. 1985 Jun 20-26;315(6021):669-72.

Lowenthal JW, Tougne C, MacDonald HR, Smith KA, Nabholz M. Antigenic stimulation regulates the expression of IL 2 receptors in a cytolytic T lymphocyte clone. J Immunol. 1985 Feb;134(2):931-9.

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Antigens	Area of Biology	Cell Type	Formats
CD25	Adaptive Immunity Cell Signaling Cytokines, Chemokines, Growth Factors	B Cells T Cells Treg Cells	Alexa Fluor 488