Anti-Mouse CD25 Alexa Fluor® 488

Also known as: Interleukin-2 Receptor alpha, IL-2Ra, IL2Ra, Tac, Ly-43

Clone: PC61.5

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-0251

Cat. No. Size
53-0251-80 25 ug
53-0251-82 100 ug

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Data for Anti-Mouse CD25 Alexa Fluor® 488.

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  • Data for Anti-Mouse CD25 Alexa Fluor® 488.
Description

Description: The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Rα). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

Details
Host Rat
Isotype IgG1, lambda
Reactivity Mouse
Conjugate Alexa Fluor 488
Laser Blue Laser
Emit 519 nm
Excite 488 nm
Legal Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications Flow Cytometric Analysis
Documentation

For complete product information, please download the TDS or IFU document.

TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
17-0251 Anti-Mouse CD25 APC 633 nm 660 nm FC RUO
47-0251 Anti-Mouse CD25 APC-eFluor® 780 633 nm 780 nm FC RUO
45-0251 Anti-Mouse CD25 PerCP-Cy5.5 488 nm 690 nm FC RUO
48-0251 Anti-Mouse CD25 eFluor® 450 405 nm 450 nm FC RUO
12-0251 Anti-Mouse CD25 PE 488 nm 575 nm FC RUO
15-0251 Anti-Mouse CD25 PE-Cy5 488 nm 670 nm FC RUO
25-0251 Anti-Mouse CD25 PE-Cy7 488 nm 760 nm FC RUO
56-0251 Anti-Mouse CD25 Alexa Fluor® 700 633 nm 723 nm FC RUO
14-0251 Anti-Mouse CD25 Purified FC RUO
16-0251 Anti-Mouse CD25 Functional Grade Purified FC, FA RUO
13-0251 Anti-Mouse CD25 Biotin FC RUO
36-0251 Anti-Mouse CD25 Functional Grade Biotin FC RUO
References

References: Tuve S, Chen BM, Liu Y, Cheng TL, Touré P, Sow PS, Feng Q, Kiviat N, Strauss R, Ni S, Li ZY, Roffler SR, Lieber A. Combination of tumor site-located CTL-associated antigen-4 blockade and systemic regulatory T-cell depletion induces tumor-destructive immune responses. Cancer Res. 2007 Jun 15;67(12):5929-39. (PC61.5, in vivo depletion, PubMed)

Huang B, Zhao J, Shen S, Li H, He KL, Shen GX, Mayer L, Unkeless J, Li D, Yuan Y, Zhang GM, Xiong H, Feng ZH. Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling. Cancer Res. 2007 May 1;67(9):4346-52. (PC61.5, in vivo depletion, PubMed)

Hayashi T, Hasegawa K, Adachi C. Elimination of CD4+CD25+ T cell accelerates the development of glomerulonephritis during the preactive phase in autoimmune-prone female NZB × NZW F1 mice. Int J Exp Pathol. 2005 Oct;86(5):289-96. (PC61.5, in vivo depletion, PubMed)

Lowenthal JW, Corthésy P, Tougne C, Lees R, MacDonald HR, Nabholz M. High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61. J Immunol. 1985 Dec;135(6):3988-94.

Lowenthal JW, Zubler RH, Nabholz M, MacDonald HR. Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes. Nature. 1985 Jun 20-26;315(6021):669-72.

Lowenthal JW, Tougne C, MacDonald HR, Smith KA, Nabholz M. Antigenic stimulation regulates the expression of IL 2 receptors in a cytolytic T lymphocyte clone. J Immunol. 1985 Feb;134(2):931-9.