Anti-Mouse CD25 Alexa Fluor® 488

Also known as: Interleukin-2 Receptor alpha

Clone: eBio7D4 (7D4)

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-0252

Cat. No.	Size
53-0252-80	25 ug
53-0252-82	100 ug
Please inquire about custom configurations or bulk packaging.

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Staining of BALB/c splenocytes with Anti-Mouse CD4 PE (cat. 12-0042) and 0.06 ug of Rat IgM kappa...View More

Description

Description: The eBio7D4 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage, as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) followed by subsequent detection with another one. eBio7D4 has been reported to block IL-2 binding but can be overcome by additional IL-2. The monoclonal antibody PC61.5 (cat. 16-0251) is recommended for in vivo studies.

Details

Host	Rat
Isotype	IgM, kappa
Reactivity	Mouse
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
50-0252	Anti-Mouse CD25 eFluor® 660	633 nm	668 nm	FC	RUO
13-0252	Anti-Mouse CD25 Biotin			FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
12-0042	Anti-Mouse CD4 PE	488 - 561 nm	575 nm	FC	RUO
53-0251	Anti-Mouse CD25 Alexa Fluor® 488	488 nm	519 nm	FC	RUO
53-0253	Anti-Mouse CD25 Alexa Fluor® 488	488 nm	519 nm	FC	RUO
53-0259	Anti-Human CD25 Alexa Fluor® 488	488 nm	519 nm	FC	RUO
53-4341	Rat IgM Isotype Control Alexa Fluor® 488	488 nm	519 nm	FC	RUO

References

References: Malek TR, Robb RJ, Shevach EM.Identification and initial characterization of a rat monoclonal antibody reactive with the murine interleukin 2 receptor-ligand complex. Proc Natl Acad Sci U S A. 1983 Sep;80(18):5694-8.

Ortega G, Robb RJ, Shevach EM, Malek TR.The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells. J Immunol. 1984 Oct;133(4):1970-5.

View More Products

Antigens	Area of Biology	Cell Type	Formats
CD25	Adaptive Immunity Cell Signaling Cytokines, Chemokines, Growth Factors	B Cells T Cells Treg Cells	Alexa Fluor 488