Anti-Mouse CD25 Alexa Fluor® 488

Also known as: Interleukin-2 Receptor alpha, IL-2Ra, IL2Ra, Tac, Ly-43

Clone: eBio3C7 (3C7)

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-0253

Cat. No.	Size
53-0253-80	25 ug
53-0253-82	100 ug
Please inquire about custom configurations or bulk packaging.

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Staining of BALB/c splenocytes with Anti-Mouse CD4 PE (cat. 12-0042) and 0.06 ug of Rat IgG2b kappa...View More

Description

Description: The eBio3C7 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of the IL-2 receptor is inhibited by the 3C7 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) and subsequent detection with another CD25 antibody.

Details

Host	Rat
Isotype	IgG2b, kappa
Reactivity	Mouse
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
48-0253	Anti-Mouse CD25 eFluor® 450	405 nm	450 nm	FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
12-0042	Anti-Mouse CD4 PE	488 - 561 nm	575 nm	FC	RUO
53-0251	Anti-Mouse CD25 Alexa Fluor® 488	488 nm	519 nm	FC	RUO
53-0252	Anti-Mouse CD25 Alexa Fluor® 488	488 nm	519 nm	FC	RUO
53-0259	Anti-Human CD25 Alexa Fluor® 488	488 nm	519 nm	FC	RUO
53-4031	Rat IgG2b K Isotype Control Alexa Fluor® 488	488 nm	519 nm	FC, ICC, IHC	RUO

References

References: Klug DB, Carter C, Crouch E, Roop D, Conti CJ, Richie ER. Interdependence of cortical thymic epithelial cell differentiation and T-lineage commitment. Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11822-7. (eBio3C7, IH/F, PubMed)

Ortega G, Robb RJ, Shevach EM, Malek TR. The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells.J Immunol. 1984 Oct;133(4):1970-5.

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Antigens	Area of Biology	Cell Type	Formats
CD25	Adaptive Immunity Cell Signaling Cytokines, Chemokines, Growth Factors	B Cells T Cells Treg Cells	Alexa Fluor 488