Anti-Mouse CD24 PE-Cyanine5

Also known as: Heat Stable Antigen, HSA

Clone: M1/69

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 15-0242

Cat. No.	Size
15-0242-81	50 ug
15-0242-82	100 ug
Please inquire about custom configurations or bulk packaging.

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Staining of BALB/c splenocytes with 0.015 ug of Rat IgG2b K Isotype Control PE-Cyanine5 (cat....View More

Description

Description: The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations. The expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.

Details

Host	Rat
Isotype	IgG2b, kappa
Reactivity	Mouse
Conjugate	PE-Cyanine5
Laser	Blue Laser, Green Laser, Yellow-Green Laser
Excite	488 - 561 nm
Legal	FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT # 5,268,486, 5,569,587 AND 5,627,027 AND FOREIGN EQUIVALENTS AND PENDING APPLICATIONS. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE. COMMERCIAL USE shall include: 1. sale, lease, license or other transfer of the material or any material derived or produced from it; 2. sale, lease, license or other grant of rights to use this Material or any material derived or produced from it; 3. use of this material to perform services for a fee for third parties. IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO EBIOSCIENCE, INC. 10255 SCIENCE CENTER DRIVE, SAN DIEGO, CALIFORNIA 92121 USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
12-0242	Anti-Mouse CD24 PE	488 - 561 nm	575 nm	FC	RUO
14-0242	Anti-Mouse CD24 Purified			FC, IHC, FA, WB	RUO
93-0242	Anti-Mouse CD24 eFluor® 605NC	350 - 590 nm	605 nm	FC	RUO
11-0242	Anti-Mouse CD24 FITC	488 nm	518 nm	FC	RUO
47-0242	Anti-Mouse CD24 APC-eFluor® 780	633 - 647 nm	780 nm	FC	RUO
16-0242	Anti-Mouse CD24 Functional Grade Purified			FC, FA	RUO
46-0242	Anti-Mouse CD24 PerCP-eFluor® 710	488 nm	710 nm	FC	RUO
25-0242	Anti-Mouse CD24 PE-Cyanine7	488 - 561 nm	775 nm	FC	RUO
13-0242	Anti-Mouse CD24 Biotin			FC	RUO
45-0242	Anti-Mouse CD24 PerCP-Cyanine5.5	488 nm		FC	RUO
48-0242	Anti-Mouse CD24 eFluor® 450	405 nm	450 nm	FC	RUO
17-0242	Anti-Mouse CD24 APC	633 - 647 nm	660 nm	FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
12-0031	Anti-Mouse CD3e PE	488 - 561 nm	575 nm	FC	RUO
12-0241	Anti-Mouse CD24 PE	488 - 561 nm	575 nm	FC	RUO
15-4031	Rat IgG2b K Isotype Control PE-Cyanine5	488 - 561 nm		FC	RUO

References

References: Hunte BE, Capone M, Zlotnik A, Rennick D, Moore TA. 1998. Acquisition of CD24 expression by Lin-CD43+B220(low)ckit(hi) cells coincides with commitment to the B cell lineage. Eur J Immunol. 28(11):3850-6.

Wilson, A., L. M. Day, et al. 1988. Subpopulations of mature murine thymocytes: properties of CD4-CD8+ and CD4+CD8- thymocytes lacking the heat-stable antigen. Cell Immunol 117(2): 312-26.

Alterman, L. A., I. N. Crispe, et al. 1990. Characterization of the murine heat-stable antigen: an hematolymphoid differentiation antigen defined by the J11d, M1/69 and B2A2 antibodies. Eur J Immunol 20(7): 1597-602.

Springer T, Galfre G, Secher DS, Milstein C. 1978. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 8(8):539-51.

View More Products

Antigens	Area of Biology	Cell Type	Formats
CD24	Adaptive Immunity	B Cells Dendritic Cells Macrophage & Monocyte Cells T Cells	PE-Cyanine5