Anti-Mouse CD22 PE (To Be Discontinued)

Description: The 2D6 monoclonal antibody reacts with mouse CD22, which is a member of the Siglec (sialic acid-binding immunoglobulin-like lectin) family. CD22 is a 150 kDa type I membrane protein expressed by the B-cell lineage in a developmentally-regulated pattern. In mouse, CD22 is expressed at low levels on the surface of pre-B cells and immature (B220^loIgM^hi) B cells, but high levels on mature (B220^hiIgM^intIgD⁺) B cells in the bone marrow. In the periphery, mouse CD22 is highly expressed on all B-cell subsets. Furthermore, mouse CD22 expression is up-regulated slightly upon activation, however expression is lost upon differentiation to a plasma cell. CD22 plays a role in the negative regulation of B-cell Receptor (BCR) signaling, however it has also been demonstrated to function as an intercellular adhesion molecule through binding of sialic acid-bearing proteins expressed by lymphocytes, neutrophils, monocytes and non-hematopoietic cells.

References: Symington FW, Subbarao B, Mosier DE, Sprent J. Lyb-8.2: A new B cell antigen defined and characterized with a monoclonal antibody.Immunogenetics. 1982;16(5):381-91.

Torres RM, Law CL, Santos-Argumedo L, Kirkham PA, Grabstein K, Parkhouse RM, Clark EA. Identification and characterization of the murine homologue of CD22, a B lymphocyte-restricted adhesion molecule.J Immunol. 1992 Oct 15;149(8):2641-9.

Doody GM, Justement LB, Delibrias CC, Matthews RJ, Lin J, Thomas ML, Fearon DT. A role in B cell activation for CD22 and the protein tyrosine phosphatase SHP.Science. 1995 Jul 14;269(5221):242-4.