Anti-Mouse CD209a (DC-SIGN) eFluor® 660

Description: This MMD3 monoclonal antibody recognizes mouse CD209a, which is also known as DC-SIGN. CD209a is a type II transmembrane C-type lectin expressed on a subset of dendritic cells, including some CD4+, CD8- and plasmacytoid pre-dendritic cells. Studies indicate that CD209a expression can vary according to the activation state of the host. Moreover, CD209a is down-regulated in spleen-derived dendritic cell cultures supplemented with GM-CSF. CD209a is involved in mediating the innate immune response by binding microbial carbohydrates.

Cross-blocking studies suggest that MMD3 recognizes a different a different epitope from LWC06 and 5H10.

References: Cheong C, Matos I, Choi JH, Schauer JD, Dandamudi DB, Shrestha E, Makeyeva JA, Li X, Li P, Steinman RM, Park CG. New monoclonal anti-mouse DC-SIGN antibodies reactive with acetone-fixed cells. J Immunol Methods. 2010 Aug 31;360(1-2):66-75. (MMD3, FC, IHC, PubMed)

Engering A, Geijtenbeek TB, van Vliet SJ, Wijers M, van Liempt E, Demaurex N, Lanzavecchia A, Fransen J, Figdor CG, Piguet V, van Kooyk Y. The dendritic cell-specific adhesion receptor DC-SIGN internalizes antigen for presentation to T cells. J Immunol. 2002 Mar 1;168(5):2118-26.

Geijtenbeek TB, Engering A, Van Kooyk Y. DC-SIGN, a C-type lectin on dendritic cells that unveils many aspects of dendritic cell biology. J Leukoc Biol. 2002 Jun;71(6):921-31.

Park CG, Takahara K, Umemoto E, Yashima Y, Matsubara K, Matsuda Y, Clausen BE, Inaba K, Steinman RM. Five mouse homologues of the human dendritic cell C-type lectin, DC-SIGN. Int Immunol. 2001 Oct;13(10):1283-90.

Caminschi I, Lucas KM, O'Keeffe MA, Hochrein H, Laâbi Y, Brodnicki TC, Lew AM, Shortman K, Wright MD. Molecular cloning of a C-type lectin superfamily protein differentially expressed by CD8alpha(-) splenic dendritic cells. Mol Immunol. 2001 Sep;38(5):365-73.