Anti-Mouse CD207 (Langerin) PE

Description: The eBioL31 is a monoclonal antibody that reacts with mouse Langerin (CD207) which is expressed in a distinct subset of dendritic cells called Langerhans cells (LC). Mouse Langerin is a 48kDa C-type lectin transmembrane protein that likely plays a role in antigen recognition and uptake. Langerhans cells are located in the epidermis and upon activation, reduce Langerin expression and begin migrating through the dermis towards lymphatic vessels. Expression of mouse Langerin in LC has been correlated to the presence of Birbeck Granules. Furthermore, the formation of Birbeck Granules has been observed upon transfection of Langerin cDNA into fibroblast cell lines.

Western Blotting with eBioL31 reveals a 48kDa band in lysate from mouse ear epidermis, as well as in lysates from cells transfected with mouse Langerin cDNA. Preliminary data from mouse Langerin cDNA transfected cells suggests that eBioL31 can be used for intracellular flow cytometric analysis in combination with the Foxp3 bufer set (cat 00-5523). The epitope recognized by eBioL31 is in the extracellular domain within the CRD (carbohydrate recognition domain) and therefore can be used to detect both surface and intracellular langerin. Differences in expression has been observed between mouse strains.

References: Idoyaga J, Suda N, Suda K, Park CG, Steinman RM. Antibody to Langerin/CD207 localizes large numbers of CD8alpha+ dendritic cells to the marginal zone of mouse spleen. Proc Natl Acad Sci U S A. 2009 Feb 3;106(5):1524-9. (L31, FC, IHC frozen sections, PubMed)

Cheong C, Idoyaga J, Do Y, Pack M, Park SH, Lee H, Kang YS, Choi JH, Kim JY, Bonito A, Inaba K, Yamazaki S, Steinman RM, Park CG. Production of monoclonal antibodies that recognize the extracellular domain of mouse Langerin/CD207.J Immunol Methods. 2008 Feb 29;331(1-2):27-38. (eBioL31, FC, IC, IP, IHC, WB PubMed)

Stoitzner P, Holzman , McLellan AD, Ivarsson L, Stossel H, Kapp M, Kammerer U, Douillard P, Kampgen E, Koch F, Saeland S, Romani N. Visualization and characterization of migratory Langerhans cells in murine skin and lymph nodes by antibodies against Langerin/CD207. J Invest Dermatol. 2003 Feb;120(2):266-74.

Valladeau J, Clair-Moninot V, Dezutter-Dambuyant C, Pin JJ, Kissenpfennig A, Mattei MG, Ait-Yahia S, Bates EE, Malissen B, Koch F, Fossiez F, Romani N, Lebecque S, Saeland S. Identification of mouse langerin/CD207 in Langerhans cells and some dendritic cells of lymphoid tissues. J Immunol. 2002 Jan 15;168(2):782-92.