Anti-Mouse CD205 Alexa Fluor® 488

Also known as: DEC-205, Ly75

Clone: 205yekta

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-2051

Cat. No.	Size
53-2051-80	25 ug
53-2051-82	100 ug
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Staining of C57Bl/6 bone marrow cells with Anti-Mouse Ly-6G (Gr-1) PE (cat. 12-5931) and 0.125 ug of...View More

Description

Description: The 205yekta antibody reacts with mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells.

CD205 has been shown to bind to PE-Cy5.5 antibody and streptavidin conjugates; therefore, this format should not be used on CD205+ dendritic cells.

Details

Host	Rat
Isotype	IgG2a, kappa
Reactivity	Mouse
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
46-2051	Anti-Mouse CD205 PerCP-eFluor® 710	488 nm	710 nm	FC	RUO
25-2051	Anti-Mouse CD205 PE-Cyanine7	488 - 561 nm	775 nm	FC	RUO
17-2051	Anti-Mouse CD205 APC	633 - 647 nm	660 nm	FC	RUO
12-2051	Anti-Mouse CD205 PE	488 - 561 nm		FC	RUO
14-2051	Anti-Mouse CD205 Purified			FC, WB	RUO
13-2051	Anti-Mouse CD205 Biotin			FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
12-5931	Anti-Mouse Ly-6G (Gr-1) PE	488 - 561 nm	575 nm	FC	RUO
53-4321	Rat IgG2a K Isotype Control Alexa Fluor® 488	488 nm	519 nm	FC, ICC, IHC	RUO

References

References: Park CG, Rodriguez A, Steinman RM. PE-Cy5.5 conjugates bind to the cells expressing mouse DEC205/CD205. J Immunol Methods. 2012 Oct 31;384(1-2):184-90.

Bonifaz LC, Bonnyay DP, Charalambous A, Darguste DI, Fujii S, Soares H, Brimnes MK, Moltedo B, Moran TM, Steinman RM. In vivo targeting of antigens to maturing dendritic cells via the DEC-205 receptor improves T cell vaccination. J Exp Med. 2004 Mar 15;199(6):815-24.

Swiggard WJ, Mirza A, Nussenzweig MC, Steinman RM. DEC-205, a 205-kDa protein abundant on mouse dendritic cells and thymic epithelium that is detected by the monoclonal antibody NLDC-145: purification, characterization, and N-terminal amino acid sequence. Cell Immunol. 1995 Oct 15;165(2):302-11.

Witmer-Pack MD, Swiggard WJ, Mirza A, Inaba K, Steinman RM. Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. II. Expression in situ in lymphoid and nonlymphoid tissues. Cell Immunol. 1995 Jun;163(1):157-62.

Inaba K, Swiggard WJ, Inaba M, Meltzer J, Mirza A, Sasagawa T, Nussenzweig MC, Steinman RM.Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. I. Expression on dendritic cells and other subsets of mouse leukocytes. Cell Immunol. 1995 Jun;163(1):148-56.

Kraal G, Breel M, Janse M, Bruin G. Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J Exp Med. 1986 Apr 1;163(4):981-97.

View More Products

Antigens	Area of Biology	Cell Type	Formats
CD205	Innate Immunity	Dendritic Cells	Alexa Fluor 488