Anti-Mouse CD184 (CXCR4) PerCP-eFluor® 710

Description: The 2B11 monoclonal antibody reacts with mouse and human CXCR4, also known as CD184, Fusin, LESTR, or HUMSTR. Fusin is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. In addition it has been shown to play a role in lymphocyte migration. The ligand for CXCR4 is SDF1. CXCR4 is required for maintenance of granulocyte progenitors in the bone marrow and essential for B cell development. Expression of CXCR4 is different between mice and human. In the thymus CXCR4 is restricted to CD4+CD8+ cells while in the spleen predominant expression is found on B lymphocytes.

The monoclonal antibody 2B11 recognizes the amino terminus of CXCR4 and has been reported to have functional activity; it does not block SDF1a but causes internalization of the receptor.

References: Forster R, Kremmer E, Schubel A, Breitfeld D, Kleinschmidt A, Nerl C, Bernhardt G, Lipp M. Intracellular and surface expression of the HIV-1 coreceptor CXCR4/fusin on various leukocyte subsets: rapid internalization and recycling upon activation. J Immunol. 1998 Feb 1;160(3):1522-31. (2B11, FC, WB, FA, PubMed)

Schabath R, Muller G, Schubel A, Kremmer E, Lipp M, Forster R. The murine chemokine receptor CXCR4 is tightly regulated during T cell development and activation. J Leukoc Biol. 1999 Dec;66(6):996-1004.(2B11, FC, WB PubMed)

Stumm RK, Rummel J, Junker V, Culmsee C, Pfeiffer M, Krieglstein J, Hollt V, Schulz S. A dual role for the SDF-1/CXCR4 chemokine receptor system in adult brain: isoform-selective regulation of SDF-1 expression modulates CXCR4-dependent neuronal plasticity and cerebral leukocyte recruitment after focal ischemia. J Neurosci. 2002 Jul 15;22(14):5865-78. (2B11, WB, PubMed)