Anti-Mouse CD127 Functional Grade Purified

Description: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity α subunit of the mouse IL-7 receptor. IL-7 receptor α chain is expressed by immature B cells in the bone marrow, double-negative (CD4^-/CD8^-), single-positive (CD4⁺ and CD8⁺), but not double-positive (CD4⁺/CD8⁺) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.

References: Sudo, T., S. Nishikawa, et al. 1993. Expression and function of the interleukin 7 receptor in murine lymphocytes. Proc Natl Acad Sci U S A 90(19): 9125-9.

Ohana M, Okazaki K, Oshima C, et al. 2001. A critical role for IL-7R signaling in the development of Helicobacter felis-induced gastritis in mice. Gastroenterology. Aug;121(2):329-36.

Okuno Y, Iwasaki H, Huettner CS, Radomska HS, Gonzalez DA, Tenen DG, Akashi K. 2002. Differential regulation of the human and murine CD34 genes in hematopoietic stem cells. Proc Natl Acad Sci U S A. Apr 30;99(9):6246-51.

Leithauser F, Meinhardt-Krajina T, et al. 2006. Foxp3-expressing CD103+ regulatory T cells accumulate in dendritic cell aggregates of the colonic mucosa in murine transfer colitis. Am J Pathol. 168(6):1898-909. (A7R34, IHC frozen, PubMed)

LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen.Vondenhoff MF, Greuter M, Goverse G, Elewaut D, Dewint P, Ware CF, Hoorweg K, Kraal G, Mebius RE.J Immunol. 2009 May 1;182(9):5439-45. (A7R34, IF, PubMed)