Anti-Mouse CD127 Alexa Fluor® 647

Also known as: Interleukin-7 Receptor alpha, IL-7Ra, Interleukin-7 receptor subunit alpha, IL7R, IL-7R alpha

Clone: A7R34

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 51-1271

Cat. No.	Size
51-1271-80	25 ug
51-1271-82	100 ug

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Description

Description: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity α subunit of the mouse IL-7 receptor. IL-7 receptor α chain is expressed by immature B cells in the bone marrow, double-negative (CD4^-/CD8^-), single-positive (CD4⁺ and CD8⁺), but not double-positive (CD4⁺/CD8⁺) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.

Details

Host	Rat
Isotype	IgG2a, kappa
Reactivity	Mouse
Conjugate	Alexa Fluor 647
Laser	Red Laser
Emit	668 nm
Excite	633 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

For complete product information, please download the TDS or IFU document.

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
14-1271	Anti-Mouse CD127 Purified			FC, IHC, FA, IP	RUO
53-1271	Anti-Mouse CD127 Alexa Fluor® 488	488 nm	519 nm	FC	RUO
47-1271	Anti-Mouse CD127 APC-eFluor® 780	633 nm	780 nm	FC	RUO
11-1271	Anti-Mouse CD127 FITC	488 nm	518 nm	FC	RUO
56-1271	Anti-Mouse CD127 Alexa Fluor® 700	633 nm	723 nm	FC	RUO
17-1271	Anti-Mouse CD127 APC	633 nm	660 nm	FC	RUO
48-1271	Anti-Mouse CD127 eFluor® 450	405 nm	450 nm	FC	RUO
16-1271	Anti-Mouse CD127 Functional Grade Purified			FC, FA	RUO
45-1271	Anti-Mouse CD127 PerCP-Cy5.5	488 nm	690 nm	FC	RUO
13-1271	Anti-Mouse CD127 Biotin			FC	RUO
15-1271	Anti-Mouse CD127 PE-Cy5	488 nm	670 nm	FC	RUO
25-1271	Anti-Mouse CD127 PE-Cy7	488 nm	760 nm	FC	RUO
12-1271	Anti-Mouse CD127 PE	488 nm	575 nm	FC	RUO

References

References: Sudo, T., S. Nishikawa, et al. 1993. Expression and function of the interleukin 7 receptor in murine lymphocytes. Proc Natl Acad Sci U S A 90(19): 9125-9.

Ohana M, Okazaki K, Oshima C, et al. 2001. A critical role for IL-7R signaling in the development of Helicobacter felis-induced gastritis in mice. Gastroenterology. Aug;121(2):329-36.

Okuno Y, Iwasaki H, Huettner CS, Radomska HS, Gonzalez DA, Tenen DG, Akashi K. 2002. Differential regulation of the human and murine CD34 genes in hematopoietic stem cells. Proc Natl Acad Sci U S A. Apr 30;99(9):6246-51.

Leithauser F, Meinhardt-Krajina T, et al. 2006. Foxp3-expressing CD103+ regulatory T cells accumulate in dendritic cell aggregates of the colonic mucosa in murine transfer colitis. Am J Pathol. 168(6):1898-909. (A7R34, IHC frozen, PubMed)

LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen.Vondenhoff MF, Greuter M, Goverse G, Elewaut D, Dewint P, Ware CF, Hoorweg K, Kraal G, Mebius RE.J Immunol. 2009 May 1;182(9):5439-45. (A7R34, IF, PubMed)

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Antigens	Area of Biology	Cell Type	Formats
CD127	Cell Signaling Cytokines, Chemokines, Growth Factors	B Cells T Cells Treg Cells	Alexa Fluor 647