Mouse CD120b (TNF Receptor II) ELISA Ready-SET-Go!®Mouse CD120b

Also known as: TNFRSF1A, TNFR1p55, TNF RI, TNFRI, TNF receptor superfamily member 1A, TNFR55, TNFR60

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-1205

Cat. No. Size
88-1205-22 2 x 96 tests w/plates
88-1205-86 10 x 96 tests w/plates
88-1205-88 10 x 96 tests

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Data for Mouse CD120b (TNF Receptor II) ELISA Ready-SET-Go!®Mouse CD120b.

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Description

Description: This mouse TNF Receptor II (TNFRII) ELISA Ready-SET-Go! Reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of mouse TNFRII protein levels from samples including serum, plasma, and supernatants from cell cultures.

Details
Reactivity Mouse
Sensitivity 4pg/ml
Standard Curve Range 4-500pg/ml
Components Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder or 10X PBS ELISA Coating Buffer. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)
Reported Applications ELISA
Documentation

For complete product information, please download the TDS or IFU document.

TDS Link Download TDS
Additional Formats
References

References: Vielhauer V, Stavrakis G, Mayadas TN. Renal cell-expressed TNF receptor 2, not receptor 1, is essential for the development of glomerulonephritis. J Clin Invest. 2005 May;115(5):1199-209.

Sashio H, Tamura K, Ito R, Yamamoto Y, Bamba H, Kosaka T, Fukui S, Sawada K, Fukuda Y, Tamura K, Satomi M, Shimoyama T, Furuyama J. Polymorphisms of the TNF gene and the TNF receptor superfamily member 1B gene are associated with susceptibility to ulcerative colitis and Crohn's disease, respectively. Immunogenetics. 2002 Mar;53(12):1020-7.

Glenn CL, Wang WY, Benjafield AV, Morris BJ. Linkage and association of tumor necrosis factor receptor 2 locus with hypertension, hypercholesterolemia and plasma shed receptor. Hum Mol Genet. 2000 Aug 12;9(13):1943-9.

Bruce AJ, Boling W, Kindy MS, Peschon J, Kraemer PJ, Carpenter MK, Holtsberg FW, Mattson MP. Altered neuronal and microglial responses to excitotoxic and ischemic brain injury in mice lacking TNF receptors. Nat Med. 1996 Jul;2(7):788-94.