Anti-Mouse CD11c Alexa Fluor® 532

Also known as: Integrin alpha X, Integrin aX, ITGAX, p150/95, leu M5 alpha

Clone: N418

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 58-0114

Cat. No. Size
58-0114-80 25 ug
58-0114-82 100 ug
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Data for Anti-Mouse CD11c Alexa Fluor® 532.

Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) APC (cat. 17-0452) and staining...View More

  • Data for Anti-Mouse CD11c Alexa Fluor® 532.
Description

Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alpha X. CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.

Details
Host Armenian Hamster
Isotype IgG
Reactivity Mouse
Conjugate Alexa Fluor 532
Laser Green Laser
Emit 561 nm
Excite 532 nm
Legal Alexa Fluor® is a registered trademark of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
50-0114 Anti-Mouse CD11c eFluor® 660 (Alexa® 647 Replacement) 633 - 647 nm 668 nm FC RUO
48-0114 Anti-Mouse CD11c eFluor® 450 405 nm 450 nm FC RUO
IH94-0114 Anti-Mouse CD11c eFluor® 625NC (for IHC/ICC) 350 - 610 nm 625 nm ICC, IHC RUO
16-0114 Anti-Mouse CD11c Functional Grade Purified FC RUO
15-0114 Anti-Mouse CD11c PE-Cyanine5 488 - 561 nm FC RUO
94-0114 Anti-Mouse CD11c eFluor® 625NC 350 - 610 nm 625 nm FC RUO
IH95-0114 Anti-Mouse CD11c eFluor® 650NC (for IHC/ICC) 350 - 635 nm 650 nm ICC, IHC RUO
12-0114 Anti-Mouse CD11c PE 488 - 561 nm FC RUO
56-0114 Anti-Mouse CD11c Alexa Fluor® 700 633 nm 723 nm FC RUO
17-0114 Anti-Mouse CD11c APC 633 - 647 nm 660 nm FC RUO
14-0114 Anti-Mouse CD11c Purified FC, IHC, IP RUO
13-0114 Anti-Mouse CD11c Biotin FC RUO
35-0114 Anti-Mouse CD11c PE-Cyanine5.5 488 - 567 nm 690 nm FC RUO
53-0114 Anti-Mouse CD11c Alexa Fluor® 488 488 nm 519 nm FC RUO
47-0114 Anti-Mouse CD11c APC-eFluor® 780 633 - 647 nm 780 nm FC RUO
25-0114 Anti-Mouse CD11c PE-Cyanine7 488 - 561 nm 775 nm FC RUO
42-0114 Anti-Mouse CD11c eFluor® 615 ICC, IHC RUO
45-0114 Anti-Mouse CD11c PerCP-Cyanine5.5 488 nm FC RUO
11-0114 Anti-Mouse CD11c FITC 488 nm 518 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
00-4300 10X RBC Lysis Buffer (Multi-species) FC, FA GPR
17-0452 Anti-Human/Mouse CD45R (B220) APC 633 - 647 nm 660 nm FC RUO
58-4888 Armenian Hamster IgG Isotype Control Alexa Fluor® 532 532 nm 561 nm FC RUO
References

References: Xin KQ, Mizukami H, Urabe M, Toda Y, Shinoda K, Yoshida A, Oomura K, Kojima Y, Ichino M, Klinman D, Ozawa K, Okuda K. Induction of robust immune responses against human immunodeficiency virus is supported by the inherent tropism of adeno-associated virus type 5 for dendritic cells. J Virol. 2006 Dec;80(24):11899-910. (N418, FC)

Ohteki T, Tada H, Ishida K, Sato T, Maki C, Yamada T, Hamuro J, Koyasu S. Essential roles of DC-derived IL-15 as a mediator of inflammatory responses in vivo. J Exp Med. 2006 Oct 2;203(10):2329-38. N418, IHC frozen)

Zhang J, Kawashima N, Suda H, Nakano Y, Takano Y, Azuma M. The existence of CD11c+ sentinel and F4/80+ interstitial dendritic cells in dental pulp and their dynamics and functional properties. Int Immunol. 2006 Sep;18(9):1375-84. (N418, IHC frozen)

Guiducci C, Vicari AP, Sangaletti S, Trinchieri G, Colombo MP. Redirecting in vivo elicited tumor infiltrating macrophages and dendritic cells towards tumor rejection. Cancer Res. 2005 Apr 15;65(8):3437-46. (N418, IHC frozen)

Esche C, Gambotto A, Satoh Y, Gerein V, Robbins PD, Watkins SC, Lotze MT, Shurin MR. CD154 inhibits tumor-induced apoptosis in dendritic cells and tumor growth.Eur J Immunol. 1999 Jul;29(7):2148-55.

Finkelman FD, Lees A, Birnbaum R, Gause WC, Morris SC. Dendritic cells can present antigen in vivo in a tolerogenic or immunogenic fashion.J Immunol. 1996 Aug 15;157(4):1406-14.

Crowley MT, Inaba K, Witmer-Pack MD, Gezelter S, Steinman RM. Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen. J Immunol Methods. 1990 Oct 4;133(1):55-66.

Metlay JP, Witmer-Pack MD, Agger R, Crowley MT, Lawless D, Steinman RM. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J Exp Med. 1990 May 1;171(5):1753-71.