Anti-Mouse CD11c Alexa Fluor® 488

Also known as: p150, integrin αX, Itgax, CR4

Clone: N418

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-0114

Cat. No. Size
53-0114-80 25 ug
53-0114-82 100 ug

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Data for Anti-Mouse CD11c Alexa Fluor® 488.

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  • Data for Anti-Mouse CD11c Alexa Fluor® 488.
Description

Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin αX. CD11c non-covalently associates with β2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.

Details
Host Armenian Hamster
Isotype IgG
Reactivity Mouse
Conjugate Alexa Fluor 488
Laser Blue Laser
Emit 519 nm
Excite 488 nm
Legal Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications Flow Cytometric Analysis
Documentation

For complete product information, please download the TDS or IFU document.

TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
94-0114 Anti-Mouse CD11c eFluor® 625NC 350 - 610 nm 625 nm FC RUO
IH95-0114 Anti-Mouse CD11c eFluor® 650NC (for IHC/ICC) 350 - 635 nm 650 nm ICC, IHC RUO
50-0114 Anti-Mouse CD11c eFluor® 660 (Alexa® 647 Replacement) 633 nm 668 nm FC RUO
IH94-0114 Anti-Mouse CD11c eFluor® 625NC (for IHC/ICC) 350 - 610 nm 625 nm ICC, IHC RUO
17-0114 Anti-Mouse CD11c APC 633 nm 660 nm FC RUO
16-0114 Anti-Mouse CD11c Functional Grade Purified FC RUO
48-0114 Anti-Mouse CD11c eFluor® 450 405 nm 450 nm FC RUO
14-0114 Anti-Mouse CD11c Purified FC, IHC, IP RUO
42-0114 Anti-Mouse CD11c eFluor® 615 ICC, IHC RUO
12-0114 Anti-Mouse CD11c PE 488 nm 575 nm FC RUO
47-0114 Anti-Mouse CD11c APC-eFluor® 780 633 nm 780 nm FC RUO
56-0114 Anti-Mouse CD11c Alexa Fluor® 700 633 nm 723 nm FC RUO
35-0114 Anti-Mouse CD11c PE-Cy5.5 488 nm 690 nm FC RUO
25-0114 Anti-Mouse CD11c PE-Cy7 488 nm 760 nm FC RUO
15-0114 Anti-Mouse CD11c PE-Cy5 488 nm 670 nm FC RUO
11-0114 Anti-Mouse CD11c FITC 488 nm 518 nm FC RUO
45-0114 Anti-Mouse CD11c PerCP-Cy5.5 (PE-Cy5.5 replacement) 488 nm 690 nm FC RUO
51-0114 Anti-Mouse CD11c Alexa Fluor® 647 (To Be Discontinued. Refer to Replacement Format® eFluor 660, cat. 50-0114) 633 nm 668 nm FC RUO
13-0114 Anti-Mouse CD11c Biotin FC RUO
References

References: Xin KQ, Mizukami H, Urabe M, Toda Y, Shinoda K, Yoshida A, Oomura K, Kojima Y, Ichino M, Klinman D, Ozawa K, Okuda K. Induction of robust immune responses against human immunodeficiency virus is supported by the inherent tropism of adeno-associated virus type 5 for dendritic cells. J Virol. 2006 Dec;80(24):11899-910. (N418, FC, PubMed)

Ohteki T, Tada H, Ishida K, Sato T, Maki C, Yamada T, Hamuro J, Koyasu S. Essential roles of DC-derived IL-15 as a mediator of inflammatory responses in vivo. J Exp Med. 2006 Oct 2;203(10):2329-38. (N418, IHC frozen, PubMed)

Zhang J, Kawashima N, Suda H, Nakano Y, Takano Y, Azuma M. The existence of CD11c+ sentinel and F4/80+ interstitial dendritic cells in dental pulp and their dynamics and functional properties. Int Immunol. 2006 Sep;18(9):1375-84. (N418, IHC frozen, PubMed)

Guiducci C, Vicari AP, Sangaletti S, Trinchieri G, Colombo MP. Redirecting in vivo elicited tumor infiltrating macrophages and dendritic cells towards tumor rejection. Cancer Res. 2005 Apr 15;65(8):3437-46. (N418, IHC frozen, PubMed)

Esche C, Gambotto A, Satoh Y, Gerein V, Robbins PD, Watkins SC, Lotze MT, Shurin MR. CD154 inhibits tumor-induced apoptosis in dendritic cells and tumor growth.Eur J Immunol. 1999 Jul;29(7):2148-55.

Finkelman FD, Lees A, Birnbaum R, Gause WC, Morris SC. Dendritic cells can present antigen in vivo in a tolerogenic or immunogenic fashion.J Immunol. 1996 Aug 15;157(4):1406-14.

Crowley MT, Inaba K, Witmer-Pack MD, Gezelter S, Steinman RM. Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen. J Immunol Methods. 1990 Oct 4;133(1):55-66.

Metlay JP, Witmer-Pack MD, Agger R, Crowley MT, Lawless D, Steinman RM. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J Exp Med. 1990 May 1;171(5):1753-71.