Anti-Mouse CD11b eFluor® 450

Also known as: Integrin alpha M, ITGAM, Mac-1 alpha (Mac1A), Complement Receptor 3 alpha (CR3A)

Clone: M1/70

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 48-0112

Cat. No. Size
48-0112-80 25 ug
48-0112-82 100 ug
Please inquire about custom configurations or bulk packaging.

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Data for Anti-Mouse CD11b eFluor® 450 (Pacific Blue® replacement).

Staining of BALB/c bone marrow cells with 0.06 ug of Anti-Mouse CD11b eFluor® 450 (cat. 48-0112). ...View More

  • Data for Anti-Mouse CD11b eFluor® 450 (Pacific Blue® replacement).
Description

Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin αM. CD11b non-covalently associates with CD18 to form αMβ2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.

Details
Host Rat
Isotype IgG2b, kappa
Reactivity Mouse
Conjugate eFluor 450
Laser Violet Laser
Excite 405 nm
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
19-0112 Anti-Mouse CD11b Cy5 633 - 647 nm 670 nm FC RUO
53-0112 Anti-Mouse CD11b Alexa Fluor® 488 488 nm 519 nm FC RUO
36-0112 Anti-Mouse CD11b Functional Grade Biotin FC, IHC, FA, IP RUO
12-0112 Anti-Mouse CD11b PE 488 - 561 nm 575 nm FC RUO
47-0112 Anti-Mouse CD11b APC-eFluor® 780 633 - 647 nm 780 nm FC RUO
56-0112 Anti-Mouse CD11b Alexa Fluor® 700 633 nm 723 nm FC RUO
15-0112 Anti-Mouse CD11b PE-Cyanine5 488 - 561 nm FC RUO
50-0112 Anti-Mouse CD11b eFluor® 660 (Alexa Fluor® 647 Replacement) 633 nm 668 nm FC RUO
16-0112 Anti-Mouse CD11b Functional Grade Purified FC, FA RUO
IH93-0112 Anti-Mouse CD11b eFluor® 605NC (for IHC/ICC) 350 - 590 nm 605 nm ICC, IHC RUO
IH95-0112 Anti-Mouse CD11b eFluor® 650NC (for IHC/ICC) 350 - 635 nm 650 nm ICC, IHC RUO
13-0112 Anti-Mouse CD11b Biotin FC RUO
45-0112 Anti-Mouse CD11b PerCP-Cyanine5.5 488 nm FC RUO
14-0112 Anti-Mouse CD11b Purified FC, IHC, FA, IP RUO
11-0112 Anti-Mouse CD11b FITC 488 nm 518 nm FC RUO
93-0112 Anti-Mouse CD11b eFluor® 605NC 350 - 590 nm 605 nm FC RUO
17-0112 Anti-Mouse CD11b APC 633 - 647 nm 660 nm FC RUO
25-0112 Anti-Mouse CD11b PE-Cyanine7 488 - 561 nm 775 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
12-4801 Anti-Mouse F4/80 Antigen PE 488 - 561 nm FC RUO
47-5931 Anti-Mouse Ly-6G (Gr-1) APC-eFluor® 780 633 - 647 nm 780 nm FC RUO
48-4031 Rat IgG2b K Isotype Control eFluor® 450 405 nm FC RUO
References

References: Zhang Y, McCormick LL, et al. 2002. Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation. J Immunol. 168(6):3088-98. (IHC frozen, PubMed)

Dembic Z, Schenck K, and Bogen B. 2000. Dendritic cells purified from myeloma are primed with tumor-specific antigen (idiotype) and activate CD4+ T cells. Proc Natl Acad Sci U S A. 97(6):2697-702. (IHC frozen, PubMed)

Whiteland JL, Nicholls SM, et al. 1995. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem. 43(3):313-20. (IHC paraffin, PubMed)

Sanchez-Madrid, F., P. Simon, et al. 1983. Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med 158(2): 586-602.

Ault KA and Springer TA. 1981. Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol. 126(1):359-64. (cross-reactivity to human, PubMed)

Springer, T., G. Galfre, et al. 1978. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol 8(8): 539-51.

Springer, T., G. Galfre, et al. 1979. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol 9(4): 301-6.