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  • Anti-Mouse CD117 (c-Kit) PE-Cyanine7 (To be Discontinued; refer to Cat. No. 25-1171)

Anti-Mouse CD117 (c-Kit) PE-Cyanine7 (To be Discontinued; refer to Cat. No. 25-1171)

Also known as: cKit, Steel Factor Receptor

Clone: ACK2

RUO: For Research Use Only. Not for use in diagnostic procedures.

Attention: Alternative mouse clone is 2B8. Refer to Cat. No. 25-1171.

SKU# 25-1172

Cat. No. Size
25-1172-82 100 ug
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Data for Anti-Mouse CD117 (c-Kit) PE-Cy7.

Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (cat. 11-0452) and 0.5...View More

  • Data for Anti-Mouse CD117 (c-Kit) PE-Cy7.
Description

Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.

Details
Host Rat
Isotype IgG2b, kappa
Reactivity Mouse
Conjugate PE-Cyanine7
Laser Blue Laser, Green Laser, Yellow-Green Laser
Emit 775 nm
Excite 488 - 561 nm
Legal FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT # 5,268,486, 5,569,587 AND 5,627,027 AND FOREIGN EQUIVALENTS AND PENDING APPLICATIONS. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE. COMMERCIAL USE shall include: 1. sale, lease, license or other transfer of the material or any material derived or produced from it; 2. sale, lease, license or other grant of rights to use this Material or any material derived or produced from it; 3. use of this material to perform services for a fee for third parties. IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO EBIOSCIENCE, INC. 10255 SCIENCE CENTER DRIVE, SAN DIEGO, CALIFORNIA 92121 USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
17-1172 Anti-Mouse CD117 (c-Kit) APC 633 - 647 nm 660 nm FC RUO
12-1172 Anti-Mouse CD117 (c-Kit) PE 488 - 561 nm 575 nm FC RUO
16-1172 Anti-Mouse CD117 (c-Kit) Functional Grade Purified FC, FA RUO
14-1172 Anti-Mouse CD117 (c-Kit) Purified FC, IHC, FA, IP, WB RUO
47-1172 Anti-Mouse CD117 (c-Kit) APC-eFluor® 780 633 - 647 nm 780 nm FC RUO
56-1172 Anti-Mouse CD117 (c-Kit) Alexa Fluor® 700 633 - 647 nm 723 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
11-0452 Anti-Human/Mouse CD45R (B220) FITC 488 nm FC RUO
25-4031 Rat IgG2b K Isotype Control PE-Cyanine7 488 - 561 nm 775 nm FC RUO
References

References: Rico-Vargas, S., et al. 1994. c-kit expresssion by B cell precursors in mouse bone marrow. J. Immunol. 152: 2845.

Ito M, Kawa Y, et al. 1999. Removal of stem cell factor or addition of monoclonal anti-c-KIT antibody induces apoptosis in murine melanocyte precursors. J Invest Dermatol. 112: 796-801.

Vincent S, Segretain D, et al. 1998. Stage-specific expression of the Kit receptor and its ligand (KL) during male gametogenesis in the mouse: a Kit-KL interaction critical for meiosis. Development. 125: 4585-93.

Sato D, Lai ZF, et al. 1996. Impairment of Kit-dependent development of interstitial cells alters contractile responses of murine intestinal tract. Am J Physiol. 271: G762-71.

Yoshida H, Kunisada T, et al. 1996. Distinct stages of melanocyte differentiation revealed by anlaysis of nonuniform pigmentation patterns. Development. 122: 1207-14.

Yoshinaga K, Nishikawa S, et al. 1991. Role of c-kit in mouse spermatogenesis: identification of spermatogonia as a specific site of c-kit expression and function. Development. 113:689-99.

Torihashi S, Ward SM, et al. 1995. c-kit-dependent development of interstitial cells and electrical activity in the murine gastrointestinal tract. Cell Tissue Res. 280:97-111.

Feng H, Sandlow JI, Sandra A. 1997. Expression and function of the c-kit proto-oncogene protein in mouse sperm. Biol Reprod. 57:194-203.

Feng H, Sandlow JI, Sandra A. 1998. The c-kit receptor and its possible signaling transduction pathway in mouse spermatozoa. Mol Reprod Dev. 49:317-26.

Prasanth SG and Ali S. 2003. Expression of proto-oncogene c-kit receptor in rats (Rattus norvegicus) and identification of a mutant mRNA transcript implicated in spermatogenic failure. DNA Cell Biol. 2003 Jul;22(7):447-56. (ACK2, rat cross-reactivity, PubMed)

Ismail RS, Okawara Y, et al. 1996. Hormonal regulation of the ligand for c-kit in the rat ovary and its effects on spontaneous oocyte meiotic maturation. Mol Reprod Dev. 1996 Apr;43(4):458-69. (ACK2, rat cross-reactivity and blocking, PubMed)

Kim HM, Shin HY, and Lee EH. 1998. Morphological alterations in rat peritoneal mast cells by stem cell factor. Immunology. 1998 Jun;94(2):242-6. (ACK2, rat cross-reactivity and blocking, PubMed)

Czechowicz A, Kraft D, Weissman IL, and Bhattacharya D. 2007. Efficient transplantation via antibody-based clearance of hematopoietic stem cell niches. Science. 23;318(5854):1296-9. (ACK2, in vivo depletion HSC, PubMed)

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