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Anti-Mouse CD117 (c-Kit) Functional Grade Purified

Also known as: SCFR, Steel Factor Receptor

Clone: ACK2

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 16-1172

Cat. No. Size
16-1172-81 50 ug
16-1172-82 100 ug
16-1172-85 500 ug

Data for Anti-Mouse CD117 (c-Kit) Functional Grade Purified.

Description

Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.

Details
Host Rat
Isotype IgG2b, kappa
Reactivity Mouse
Conjugate Functional Grade Purified (low endo, azide-free)
Reported Applications Flow Cytometric Analysis, Functional Assays
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
25-1172 Anti-Mouse CD117 (c-Kit) PE-Cyanine7 488 - 561 nm 775 nm FC RUO
47-1172 Anti-Mouse CD117 (c-Kit) APC-eFluor® 780 633 - 647 nm 780 nm FC RUO
56-1172 Anti-Mouse CD117 (c-Kit) Alexa Fluor® 700 633 - 647 nm 723 nm FC RUO
17-1172 Anti-Mouse CD117 (c-Kit) APC 633 - 647 nm 660 nm FC RUO
12-1172 Anti-Mouse CD117 (c-Kit) PE 488 - 561 nm 578 nm FC RUO
14-1172 Anti-Mouse CD117 (c-Kit) Purified FC, IHC, IHC-F, FA, IP, WB RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
16-4031 Rat IgG2b K Isotype Control Functional Grade Purified FC RUO
References

References: Rico-Vargas, S., et al. 1994. c-kit expresssion by B cell precursors in mouse bone marrow. J. Immunol. 152: 2845.

Ito M, Kawa Y, et al. 1999. Removal of stem cell factor or addition of monoclonal anti-c-KIT antibody induces apoptosis in murine melanocyte precursors. J Invest Dermatol. 112: 796-801.

Vincent S, Segretain D, et al. 1998. Stage-specific expression of the Kit receptor and its ligand (KL) during male gametogenesis in the mouse: a Kit-KL interaction critical for meiosis. Development. 125: 4585-93.

Sato D, Lai ZF, et al. 1996. Impairment of Kit-dependent development of interstitial cells alters contractile responses of murine intestinal tract. Am J Physiol. 271: G762-71.

Yoshida H, Kunisada T, et al. 1996. Distinct stages of melanocyte differentiation revealed by anlaysis of nonuniform pigmentation patterns. Development. 122: 1207-14.

Yoshinaga K, Nishikawa S, et al. 1991. Role of c-kit in mouse spermatogenesis: identification of spermatogonia as a specific site of c-kit expression and function. Development. 113:689-99.

Torihashi S, Ward SM, et al. 1995. c-kit-dependent development of interstitial cells and electrical activity in the murine gastrointestinal tract. Cell Tissue Res. 280:97-111.

Feng H, Sandlow JI, Sandra A. 1997. Expression and function of the c-kit proto-oncogene protein in mouse sperm. Biol Reprod. 57:194-203.

Feng H, Sandlow JI, Sandra A. 1998. The c-kit receptor and its possible signaling transduction pathway in mouse spermatozoa. Mol Reprod Dev. 49:317-26.

Prasanth SG and Ali S. 2003. Expression of proto-oncogene c-kit receptor in rats (Rattus norvegicus) and identification of a mutant mRNA transcript implicated in spermatogenic failure. DNA Cell Biol. 2003 Jul;22(7):447-56. (ACK2, rat cross-reactivity, PubMed)

Ismail RS, Okawara Y, et al. 1996. Hormonal regulation of the ligand for c-kit in the rat ovary and its effects on spontaneous oocyte meiotic maturation. Mol Reprod Dev. 1996 Apr;43(4):458-69. (ACK2, rat cross-reactivity and blocking, PubMed)

Kim HM, Shin HY, and Lee EH. 1998. Morphological alterations in rat peritoneal mast cells by stem cell factor. Immunology. 1998 Jun;94(2):242-6. (ACK2, rat cross-reactivity and blocking, PubMed)

Czechowicz A, Kraft D, Weissman IL, and Bhattacharya D. 2007. Efficient transplantation via antibody-based clearance of hematopoietic stem cell niches. Science. 23;318(5854):1296-9. (ACK2, in vivo depletion HSC, PubMed)