Anti-Mouse CD107b (LAMP-2) Alexa Fluor® 488

Also known as: LAMP2, lysosomal-associated membrane protein 2

Clone: eBioABL-93 (ABL-93)

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-1072

Cat. No.	Size
53-1072-80	25 ug
53-1072-82	100 ug
Please inquire about custom configurations or bulk packaging.

To enable ordering, please select your country. Cookies will need to be enabled to continue on our website. View our Privacy Policy.

Intracellular staining of thioglycolate-induced peritoneal exudate cells with 0.5 ug of Rat IgG2a K...View More

Description

Description: The eBioABL-93 monoclonal antibody reacts with mouse CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2). CD107b is a highly glycosylated protein of approximately 120 kDa that is expressed intracellularly in lysosomal membranes. It is also transiently expressed on the surface of cytolytic T cells during degranulation, but to a lesser extent than CD107a. It has been reported that CD107b may be identical to the mouse Mac-3 antigen.

Details

Host	Rat
Isotype	IgG2a, kappa
Reactivity	Mouse
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Intracellular Staining Followed by Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
11-1072	Anti-Mouse CD107b (LAMP-2) FITC	488 nm	518 nm	FC	RUO
14-1072	Anti-Mouse CD107b (LAMP-2) Purified			FC, IHC, IP	RUO
50-1072	Anti-Mouse CD107b (LAMP-2) eFluor® 660 (Alexa Fluor® 647 Replacement)	633 nm	668 nm	FC	RUO
13-1072	Anti-Mouse CD107b (LAMP-2) Biotin			FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
53-1071	Anti-Mouse CD107a (LAMP-1) Alexa Fluor® 488	488 nm	519 nm	FC	RUO
53-1078	Anti-Human CD107b (LAMP-2) Alexa Fluor® 488	488 nm	519 nm	FC	RUO
53-4321	Rat IgG2a K Isotype Control Alexa Fluor® 488	488 nm	519 nm	FC, ICC, IHC	RUO
88-8823	Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A) (previously named IC Fixation & Permeabilization Buffer)			FC	GPR

References

References: Betts MR, Koup RA. Detection of T-cell degranulation: CD107a and b. Methods Cell Biol. 2004;75:497-512.

Tritel M, Stoddard AM, Flynn BJ, Darrah PA, Wu CY, Wille U, Shah JA, Huang Y, Xu L, Betts MR, Nabel GJ, Seder RA. Prime-boost vaccination with HIV-1 Gag protein and cytosine phosphate guanosine oligodeoxynucleotide, followed by adenovirus, induces sustained and robust humoral and cellular immune responses. J Immunol. 2003 Sep 1;171(5):2538-47. (ABL-93, degranulation assay, PubMed)

Cha Y, Holland SM, August JT. The cDNA sequence of mouse LAMP-2. Evidence for two classes of lysosomal membrane glycoproteins. J Biol Chem. 1990 Mar 25;265(9):5008-13. (ABL-93, affinity-chromatography, PubMed)

Chen JW, Chen GL, D'Souza MP, Murphy TL, August JT. Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2. Biochem Soc Symp. 1986;51:97-112.

Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985 Jul;101(1):85-95. (ABL-93, IP, IHC, PubMed)

View More Products

Antigens	Area of Biology	Cell Type	Formats
CD107b (LAMP-2)	Adhesion & Migration Innate Immunity	Macrophage & Monocyte Cells NK & NKT Cells T Cells	Alexa Fluor 488