Anti-Mouse AIRE Alexa Fluor® 488

Also known as: Autoimmune Regulator

Clone: 5H12

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-5934

Cat. No.	Size
53-5934-80	25 ug
53-5934-82	100 ug
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Immunohistochemistry on frozen 5-week-old mouse thymus using 10 ug/mL of Anti-Mouse AIRE Alexa...View More

Description

Description: This 5H12 monoclonal antibody reacts with mouse Autoimmune Regulator (AIRE). This 58-kDa transcription factor is highly expressed within the perinuclear region of mature medullary thymic epithelial cells (mTECs). However, expression of AIRE protein in the periphery remains unclear despite the detection of AIRE mRNA in the lymph node, lymphoid stromal cells, monocytes, and dendritic cells. Studies demonstrate that AIRE is involved in thymocyte development, in particular the regulation of tissue-specific antigen expression during negative selection. Other biological functions for AIRE include chemokine expression and antigen presentation.

The 5H12 antibody recognizes mouse AIRE isoforms 1 and 2.

Details

Host	Rat
Isotype	IgG2c, kappa
Reactivity	Mouse
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® is a registered trademark of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis, Immunohistochemical Staining of Frozen Tissue Sections

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
14-5934	Anti-Mouse AIRE Purified			FC, IHC, IP, WB	RUO
50-5934	Anti-Mouse AIRE eFluor® 660 (Alexa Fluor® 647 Replacement)	633 - 647 nm	668 nm	FC, IHC	RUO

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References

References: Anderson MS, Su MA. Aire and T cell development. Curr Opin Immunol. 2010; 23:1-9.

Hubert FX, Kinkel SA, Webster KE, Cannon P, Crewther PE, Proeitto AI, Wu L, Heath WR, Scott HS. A specific anti-Aire antibody reveals aire expression is restricted to medullary thymic epithelial cells and not expressed in periphery. J Immunol. 2008 Mar 15;180(6):3824-32. (5H12, FC, IHC-frozen, WB)

Rossi SW, Kim MY, Leibbrandt A, Parnell SM, Jenkinson WE, Glanville SH, McConnell FM, Scott HS, Penninger JM, Jenkinson EJ, Lane PJ, Anderson G. RANK signals from CD4(+)3(-) inducer cells regulate development of Aire-expressing epithelial cells in the thymic medulla. J Exp Med. 2007 Jun 11;204(6):1267-72. (5H12, IHC-frozen)

View More Products

Antigens	Area of Biology	Formats
AIRE	Adaptive Immunity Transcription Factors	Alexa Fluor 488