Description: This FlowCytomix Simplex Kit is designed for the measurement of Monkey tPA in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Monkey Basic Kit (cat. BMS8423FF), this kit can be used to detect tPA alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.
In this kit, the antibodies are human-specific and the standards are recombinant human proteins. The antibodies are cross-reactive with non-human primate cytokines.
This kit contains bead population A5.
Tissue-type plasminogen activator (tPA) is a serine protease which occurs in blood plasma, serum, other body fluids, tissues and conditioned media of certain cultured cells. It can convert the inactive proenzyme plasminogen to the active protease plasmin. Plasmin can degrade fibrin, the matrix of a blood clot in a process known as fibrinolysis, leading to dissolution of the clot. Furthermore plasminogen activation is implicated in metastatic spread of malignant cells and in tissue remodelling.
Fibrin has been shown to accelerate the conversion of plasminogen to plasmin which is mediated by tPA. Through this pathway fibrin promotes its own degradation. Inhibitors to t-PA have been found in blood preparations, cell culture media and tissues. These plasminogen activator inhibitors -1 and -2 (PAI-1, PAI-2) react extremely rapidly with tPA, forming inactive complexes. The availability of free active tPA is regulated through this interaction.
