monkey sP-selectin Platinum ELISA

Description: The monkey sP-selectin ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of monkey sP-selectin. The monkey sP-selectin ELISA is for research use only. Not for diagnostic or therapeutic procedures.

P-selectin belongs to the selectin family of adhesion molecules. P-selectin acts as a receptor that supports binding of leukocytes to activated platelets and endothelium.

P-selectin is a 140 kDa protein that is highly glycosylated. The cDNA derived amino acid sequence predicts a molecule with a series of cysteine-rich domains.

P-selectin is located in membranes of a granules in unstimulated platelets and redistributed to the cell surface upon platelet activation. P-selectin is also present in endothelial cells in membranes of Weibel-Palade bodies and megakaryocytes.

The physiologic role of P-selectin might be the mediation of initial leukocyte adhesion to activated endothelium during acute inflammation. It may work in concert with E-selectin to direct early, regionally specific adherence of neutrophils and monocytes at sites of acute inflammation. A soluble form of P-selectin found in serum and plasma has been described which might represent a proteolytic fragment or more likely a soluble splice variant lacking the transmembrane domain.

Excessive accumulation of neutrophils on the endothelial surface accompanied by high exposure of P-selectin has been implicated in a number of inflammatory disorders, including adult respiratory distress syndrome, acute lung injury, ischemia-reperfusion injury, Gram-negative septic shock, thrombotic diseases and rheumatoid arthritis. Malignant cells were shown to express receptors for P-selectin suggesting an important role for P-selectin in tumor formation and metastasis.

References: Dunlop,L.C.; Skinner,M.P.; Bendall,L.J.; Favaloro,E.J.; Castaldi,P.A.; Gorman,J.J.; Gamble,J.R.; Vadas,M.A.; Berndt,M.C.. Characterization of GMP-140 (P-selectin) as a circulating plasma protein. J.Exp.Med. 1992;175:1147-1150. (Link)

Stenberg,P.E.; McEver,R.P.; Shuman,M.A.; Jacques,Y.V.; Bainton,D.F.. A platelet alpha-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation. J.Cell Biol. 1985;101:880-886. (Link)

Johnston,G.I.; Kurosky,A.; McEver,R.P.. Structural and biosynthetic studies of the granule membrane protein, GMP-140, from human platelets and endothelial cells. J.Biol.Chem. 1989;264:1816-1823. (Link)

Mulligan,M.S.; Polley,M.J.; Bayer,R.J.; Nunn,M.F.; Paulson,J.C.; Ward,P.A.. Neutrophil-dependent acute lung injury. Requirement for P-selectin (GMP-140). J.Clin.Invest 1992;90:1600-1607. (Link)

Watson,M.L.; Kingsmore,S.F.; Johnston,G.I.; Siegelman,M.H.; Le Beau,M.M.; Lemons,R.S.; Bora,N.S.; Howard,T.A.; Weissman,I.L.; McEver,R.P.; .. Genomic organization of the selectin family of leukocyte adhesion molecules on human and mouse chromosome 1. J.Exp.Med. 1990;172:263-272. (Link)

Geng,J.G.; Bevilacqua,M.P.; Moore,K.L.; McIntyre,T.M.; Prescott,S.M.; Kim,J.M.; Bliss,G.A.; Zimmerman,G.A.; McEver,R.P.. Rapid neutrophil adhesion to activated endothelium mediated by GMP-140. Nature 1990;343:757-760. (Link)

Johnston,G.I.; Cook,R.G.; McEver,R.P.. Cloning of GMP-140, a granule membrane protein of platelets and endothelium: sequence similarity to proteins involved in cell adhesion and inflammation. Cell 1989;56:1033-1044. (Link)

Hogg,N.. Roll, roll, roll your leucocyte gently down the vein. Immunol.Today 1992;13:113-115. (Link)

Malech,H.L.; Gallin,J.I.. Current concepts: immunology. Neutrophils in human diseases. N Engl.J.Med. 1987;317:687-694. (Link)

Hamburger,S.A.; McEver,R.P.. GMP-140 mediates adhesion of stimulated platelets to neutrophils. Blood 1990;75:550-554. (Link)

Hattori,R.; Hamilton,K.K.; Fugate,R.D.; McEver,R.P.; Sims,P.J.. Stimulated secretion of endothelial von Willebrand factor is accompanied by rapid redistribution to the cell surface of the intracellular granule membrane protein GMP-140. J.Biol.Chem. 1989;264:7768-7771. (Link)

Ushiyama,S.; Laue,T.M.; Moore,K.L.; Erickson,H.P.; McEver,R.P.. Structural and functional characterization of monomeric soluble P-selectin and comparison with membrane P-selectin. J.Biol.Chem. 1993;268:15229-15237. (Link)

Johnston,G.I.; Bliss,G.A.; Newman,P.J.; McEver,R.P.. Structure of the human gene encoding granule membrane protein-140, a member of the selectin family of adhesion receptors for leukocytes. J.Biol.Chem. 1990;265:21381-21385. (Link)

Moore,K.L.; Varki,A.; McEver,R.P.. GMP-140 binds to a glycoprotein receptor on human neutrophils: evidence for a lectin-like interaction. J.Cell Biol. 1991;112:491-499. (Link)

Berman,C.L.; Yeo,E.L.; Wencel-Drake,J.D.; Furie,B.C.; Ginsberg,M.H.; Furie,B.. A platelet alpha granule membrane protein that is associated with the plasma membrane after activation. Characterization and subcellular localization of platelet activation-dependent granule-external membrane protein. J.Clin.Invest 1986;78:130-137. (Link)

Lotan,R.; Raz,A.. Endogenous lectins as mediators of tumor cell adhesion. J.Cell Biochem. 1988;37:107-117. (Link)

Larsen,E.; Celi,A.; Gilbert,G.E.; Furie,B.C.; Erban,J.K.; Bonfanti,R.; Wagner,D.D.; Furie,B.. PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes. Cell 1989;59:305-312. (Link)

George,J.N.; Pickett,E.B.; Saucerman,S.; McEver,R.P.; Kunicki,T.J.; Kieffer,N.; Newman,P.J.. Platelet surface glycoproteins. Studies on resting and activated platelets and platelet membrane microparticles in normal subjects, and observations in patients during adult respiratory distress syndrome and cardiac surgery. J.Clin.Invest 1986;78:340-348. (Link)

Boukerche,H.; Berthier-Vergnes,O.; Tabone,E.; Dore,J.F.; Leung,L.L.; McGregor,J.L.. Platelet-melanoma cell interaction is mediated by the glycoprotein IIb-IIIa complex. Blood 1989;74:658-663. (Link)