Description: This FlowCytomix Simplex Kit is designed for the measurement of Monkey sCD-40L in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Monkey Basic Kit (cat. BMS8423FF), this kit can be used to detect sCD-40L alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.
In this kit, the antibodies are human-specific and the standards are recombinant human proteins. The antibodies are cross-reactive with non-human primate cytokines.
This kit contains bead population A3.
CD40 belongs to the TNF receptor superfamily and is a biochemical marker for the identification of activated platelets of patients with cardiovascular diseases (angina pectoris, infarction, stroke etc.)
A key role of CD40/CD40ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. CD40 Ligand is expressed on the surface of activated CD4+ T cells, basophils, and mast cells. Binding of CD40L to its receptor, CD40, on the surface of B cells stimulates B-cell proliferation, adhesion and differentiation. A soluble isoform of CD40L has been shown to exist in the circulation. This soluble molecule is a homotrimer of a 18kDa protein exhibiting full activity in B cell proliferation and differentiation assays, is able to rescue B cells from apoptosis and binds soluble CD40.
CD40L is discussed in relation to a potential role in supporting B cell tumors and it has been discovered that the molecular defect in the X-linked Hyper-IgM-Syndrome is targeted to the CD40 L gene, it is functional involved in B cell hybridomas and chronic lymphocytic leukemia as well as several autoimmune diseases.
