Description: This FlowCytomix Simplex Kit is designed for the measurement of Monkey MIP-1beta in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Monkey Basic Kit (cat. BMS8423FF), this kit can be used to detect MIP-1beta alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.
In this kit, the antibodies are human-specific and the standards are recombinant human proteins. The antibodies are cross-reactive with non-human primate cytokines.
This kit contains bead population A5.
MIP-1alpha and MIP-1beta belong to the family of cysteine-cysteine (cc) chemokines, RANTES being another prominent member thereof.
Both MIP-1alpha and MIP-1beta are not only chemoattractants but also coactivators of macrophages acting in concert with IFN-gamma as type 1 cytokines.
MIP-1alpha and MIP-1beta are distinct but highly homologous chemokines produced by a variety of host cells in response to various external stimuli and share affinity for their receptor CCR5. The roles of MIP-1alpha and MIP-1beta have been elucidated in response to their affects on cellular and humoral immune response. MIP-1alpha was shown to stimulate strong antigen specific responses, while MIP-1beta promotes antibody responses. Both macrophage inflammatory proteins are however strictly associated with type 1 immune response.
Determination of the expression levels of the MIP-1s turned out to provide important information regarding numerous diseases such as multiple myeloma, allergic asthmatic disorders, acute experimental autoimmune encephalomyelitis, HIV infection, sarcoidosis and sepsis.
