Description: This FlowCytomix Simplex Kit is designed for the measurement of Monkey MCP-1 in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Monkey Basic Kit (cat. BMS8423FF), this kit can be used to detect MCP-1 alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.
In this kit, the antibodies are human-specific and the standards are recombinant human proteins. The antibodies are cross-reactive with non-human primate cytokines.
This kit contains bead population A7.
MCP-1was characterized as a monocyte-specific chemoattractant that was later shown to attract also T lymphocytes and NK cells.
The human mature MCP-1 protein consists of 76 amino acids, derived by cleavage of a hydrophobic signal peptide from the 99 aa precursor protein. MCP-1 is mainly expressed by macrophages in response to a wide range of cytokines such as IL-6, TNF-alpha and IL-1beta, but can, upon stimulation, also be produced by a variety of cells and tissues, such as fibroblasts, endothelial cells or certain tumor cells.
Because of its target cell specificity, MCP-1 was postulated to play a pathogenic role in a variety of diseases characterized by mononuclear cell infiltration, including atherosclerosis, rheumatoid arthritis and allergic responses. In basophils, MCP-1 is highly effective as a stimulus of histamine release but has only weak chemotactic activity. Additionally it has been shown to chemoattract CD4+ and CD8+ T lymphocytes, and expression of the MCP-1 chemokine may affect HIV infection via signaling through the CCR2 receptor.