Monkey IL-8 Instant ELISA

Description: The monkey IL-8 Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of monkey IL-8. Monkey IL-8 Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Interleukin-8 selectively stimulates the ability of neutrophils and T-lymphocytes to invade injured or inflamed tissue. IL-8 also known as CXCL8 that is purified from peripheral blood monocytes is a 10 kDa protein showing at least 4 variant forms (77, 72, 70, and 69 amino acids, respectively) which differ in the length of the N-terminal and readily form dimers in solution. Two cystin-bridges are essential for the biological activity of IL-8.
Exogenous stimuli like LPS, but also IL-1, TNF-alpha and TNF-beta induce the secretion of IL-8 in a variety of different cell types including monocytes, endothelial and epithelial cells, peripheral blood mononuclear cells, dermal fibroblasts, keratinocytes, neutrophils, hepatocytes, synovial cells, and T-lymphocytes.

The activities of IL-8 are not species-specific. Human IL-8 is also active in rodent and rabbit cells and differs from all other cytokines in its ability to specifically activate neutrophil granulocytes. This chemokine activates multiple intracellular signaling pathways downstream of two cell-surface, G protein-coupled receptors (CXCR1 and CXCR2). The property of IL-8 to stimulate movement of neutrophils across endothelial monolayers in vitro supports the concept of a central role for this molecule in the accumulation of neutrophils at inflammatory lesions in vivo.

Citations: Baize,S.; Marianneau,P.; Loth,P.; Reynard,S.; Journeaux,A.; Chevallier,M.; Tordo,N.; Deubel,V.; Contamin,H.. Early and strong immune responses are associated with control of viral replication and recovery in lassa virus-infected cynomolgus monkeys. J Virol. 2009;83:5890-5903. (Link)

References: Baldwin,E.T.; Weber,I.T.; St,Charles R.; Xuan,J.C.; Appella,E.; Yamada,M.; Matsushima,K.; Edwards,B.F.; Clore,G.M.; Gronenborn,A.M.; .. Crystal structure of interleukin 8: symbiosis of NMR and crystallography. Proc.Natl.Acad.Sci.U.S.A 1991;88:502-506. (Link)

Gimbrone,M.A.,Jr.; Obin,M.S.; Brock,A.F.; Luis,E.A.; Hass,P.E.; Hebert,C.A.; Yip,Y.K.; Leung,D.W.; Lowe,D.G.; Kohr,W.J.; .. Endothelial interleukin-8: a novel inhibitor of leukocyte-endothelial interactions. Science 1989;246:1601-1603. (Link)

Van,Damme J.; Bunning,R.A.; Conings,R.; Graham,R.; Russell,G.; Opdenakker,G.. Characterization of granulocyte chemotactic activity from human cytokine-stimulated chondrocytes as interleukin 8. Cytokine 1990;2:106-111. (Link)

Smith,W.B.; Gamble,J.R.; Clark-Lewis,I.; Vadas,M.A.. Interleukin-8 induces neutrophil transendothelial migration. Immunology 1991;72:65-72. (Link)

Grob,P.M.; David,E.; Warren,T.C.; DeLeon,R.P.; Farina,P.R.; Homon,C.A.. Characterization of a receptor for human monocyte-derived neutrophil chemotactic factor/interleukin-8. J.Biol.Chem. 1990;265:8311-8316. (Link)

Martich,G.D.; Danner,R.L.; Ceska,M.; Suffredini,A.F.. Detection of interleukin 8 and tumor necrosis factor in normal humans after intravenous endotoxin: the effect of antiinflammatory agents. J.Exp.Med. 1991;173:1021-1024. (Link)

Larsen,C.G.; Anderson,A.O.; Oppenheim,J.J.; Matsushima,K.. Production of interleukin-8 by human dermal fibroblasts and keratinocytes in response to interleukin-1 or tumour necrosis factor. Immunology 1989;68:31-36. (Link)

Seitz,M.; Dewald,B.; Gerber,N.; Baggiolini,M.. Enhanced production of neutrophil-activating peptide-1/interleukin-8 in rheumatoid arthritis. J.Clin.Invest 1991;87:463-469. (Link)

Detmers,P.A.; Lo,S.K.; Olsen-Egbert,E.; Walz,A.; Baggiolini,M.; Cohn,Z.A.. Neutrophil-activating protein 1/interleukin 8 stimulates the binding activity of the leukocyte adhesion receptor CD11b/CD18 on human neutrophils. J.Exp.Med. 1990;171:1155-1162. (Link)

Kunkel,S.L.; Standiford,T.; Kasahara,K.; Strieter,R.M.. Interleukin-8 (IL-8): the major neutrophil chemotactic factor in the lung. Exp.Lung Res. 1991;17:17-23. (Link)

Baggiolini,M.; Walz,A.; Kunkel,S.L.. Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. J.Clin.Invest 1989;84:1045-1049. (Link)

Peichl,P.; Ceska,M.; Effenberger,F.; Haberhauer,G.; Broell,H.; Lindley,I.J.. Presence of NAP-1/IL-8 in synovial fluids indicates a possible pathogenic role in rheumatoid arthritis. Scand.J.Immunol. 1991;34:333-339. (Link)

Sticherling,M.; Bornscheuer,E.; Schroder,J.M.; Christophers,E.. Localization of neutrophil-activating peptide-1/interleukin-8-immunoreactivity in normal and psoriatic skin. J.Invest Dermatol. 1991;96:26-30. (Link)

Lindley,I.; Aschauer,H.; Seifert,J.M.; Lam,C.; Brunowsky,W.; Kownatzki,E.; Thelen,M.; Peveri,P.; Dewald,B.; von,Tscharner,V; .. Synthesis and expression in Escherichia coli of the gene encoding monocyte-derived neutrophil-activating factor: biological equivalence between natural and recombinant neutrophil-activating factor. Proc.Natl.Acad.Sci.U.S.A 1988;85:9199-9203. (Link)

Puchner,T.; Egarter,C.; Wimmer,C.; Lederhilger,F.; Weichselbraun,I.. Amniotic fluid interleukin-8 as a marker for intraamniotic infection. Arch.Gynecol.Obstet. 1993;253:9-14. (Link)

DeMarco,D.; Kunkel,S.L.; Strieter,R.M.; Basha,M.; Zurier,R.B.. Interleukin-1 induced gene expression of neutrophil activating protein (interleukin-8) and monocyte chemotactic peptide in human synovial cells. Biochem.Biophys.Res.Commun. 1991;174:411-416. (Link)

Matsushima,K.; Oppenheim,J.J.. Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF. Cytokine 1989;1:2-13. (Link)

Peichl,P.; Ceska,M.; Broell,H.; Effenberger,F.; Lindley,I.J.. Human neutrophil activating peptide/interleukin 8 acts as an autoantigen in rheumatoid arthritis. Ann.Rheum.Dis. 1992;51:19-22. (Link)

Elford,P.R.; Cooper,P.H.. Induction of neutrophil-mediated cartilage degradation by interleukin-8. Arthritis Rheum. 1991;34:325-332. (Link)

Tilg,H.; Ceska,M.; Vogel,W.; Herold,M.; Margreiter,R.; Huber,C.. Interleukin-8 serum concentrations after liver transplantation. Transplantation 1992;53:800-803. (Link)