MAPK Family (ERK, p38, JNK) Activation InstantOne™ ELISA

Also known as: ERK, p38, JNK

RUO: For Research Use Only. Not for use in diagnostic procedures.

Attention: The MAPK Family InstantOne ELISA contains reagents for a total of 96 wells (32 wells each for phospho-ERK, phopsho-p38, and phospho-JNK)
Please contact Technical Service for a lot specific COA

SKU# 85-86015

Cat. No.	Size
85-86015-11	96 tests
Please inquire about custom configurations or bulk packaging.

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Left: A431 cells were pre-treated with 10 uM AG1478 (an EGFR kinase inhibitor) for 3 hours (-) or...View More

Description

Description: The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated ERK 1/2 (human, mouse, hamster), p38 MAPK (human, mouse), and JNK 1/2/3 (human, mouse) in cell lysates. Detection of these phosphorylated molecules in rat is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

Extracellular signal-regulated kinase (ERK), p38, and Jun N-terminal Kinases (JNKs) are members of the Mitogen-Activated Protein Kinase (MAPK) pathway. These kinases are activated via phosphorylation of the TxY motif in response to growth factors, cellular and environmental stress, and cytokines. These kinases play roles in a variety of biological processes, including cell division, survival, differentiation, and metabolism.

This kit has been developed to recognize the following phosphorylation sites:
ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187)
p38 MAPK (Thr180/Tyr182)
JNK 1/2/3 (Thr183/Tyr185)

Details

Reactivity	Human, Mouse
Components	The MAPK Family InstantOne ELISA contains sufficient reagents for a total of 96 wells (32 wells each for phospho-ERK, phopsho-p38, and phospho-JNK) User Manual InstantOne™ Assay Plate (Part No. IOAP96) - 96 well (sealed) Cell Lysis Buffer (5X) (Part No. IOCLB1) - 8 mL Enhancer Solution (Part No. IOES1) - 0.5 mL Wash Buffer (10X) (Part No. IOWB1) - 8 mL phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187) Capture Antibody Reagent (IOPERKCAR1T) - 1 x 1.0 mL phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187) Detection Antibody Reagent (IOPERKDAR1T) - 1 x 1.0 mL phospho-p38 MAPK (Thr180/Tyr182) Capture Antibody Reagent (IOPP38CAR1T) - 1 x 1.0 mL phospho-p38 MAPK (Thr180/Tyr182) Detection Antibody Reagent (IOPP38DAR1T) - 1 x 1.0 mL phospho-JNK 1/2/3 (Thr183/Tyr185) Capture Antibody Reagent (IOPJNKCAR1T) - 1 x 1.0 mL phospho-JNK 1/2/3 (Thr183/Tyr185) Detection Antibody Reagent (IOPJNKDAR1T) - 1 x 1.0 mL Positive Control Cell Lysate lyophilized (IOMMAPKPC1)- 0.25 mL Detection Reagent (TMB Substrate) (Part No. IODR1) - 10 mL Stop Solution (Part No. IOSS1) - 10 mL Plate Seal - 1 ea
Reported Applications	ELISA

Documentation

TDS Link	Download TDS
User Manual	Download User Manual

Related Products

Cat. No.	Name	Application	Reg.
85-86011	ERK 1/2 (Total) InstantOne™ ELISA	ELISA	RUO
85-86012	phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187) InstantOne™ ELISA	ELISA	RUO
85-86021	p38 MAPK (Total) InstantOne™ ELISA	ELISA	RUO
85-86022	phospho-p38 MAPK (Thr180/Tyr182) InstantOne™ ELISA	ELISA	RUO
85-86031	JNK 1/2/3 (Total) InstantOne™ ELISA	ELISA	RUO
85-86032	phospho-JNK 1/2/3 (Thr183/Tyr185) InstantOne™ ELISA	ELISA	RUO
IOCLB	InstantOne ELISA Cell Lysis Buffer (5X)	ELISA	RUO
IOES	InstantOne ELISA Enhancer Solution	ELISA	RUO
IOWB	InstantOne ELISA Wash Buffer(10X)	ELISA	RUO

References

View More Products

Antigens	Area of Biology	Cell Type
p38 ERK 1 ERK 2 JNK1 JNK3	Cell Signaling	B Cells Dendritic Cells NK & NKT Cells T Cells