Anti-Human LAP (Latency Associated Peptide) Alexa Fluor® 488

Also known as: Pro-TGF beta 1, LAP/TGF beta 1

Clone: TW4-2F8

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-9828

Cat. No.	Size
53-9828-41	25 tests
53-9828-42	100 tests
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Normal human peripheral blood cells were stimulated for 1 day with Anti-Human CD3 and Anti-Human...View More

Description

Description: The TW4-2F8 monoclonal antibody reacts with human latency associated peptide (LAP, pro-TGF beta 1, LAP/TGF beta 1). Many different cells produce TGF beta and it mediates effects on the proliferation, differentiation and function of many cell types. TGF beta is synthesized as a precursor that contains LAP at the N-terminus and mature TGF beta at the C-terminus. Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex. LAP is secreted and can be found in the extracellular matrix. In addition, LAP can also be expressed on platelets and activated regulatory T cells. It is believed that this surface-expressed LAP is due to the binding of LAP to GARP (LRRC32), which is a transmembrane protein that is also found at high levels on platelets and activated regulatory T cells.

Details

Host	Mouse
Isotype	IgG1, kappa
Reactivity	Human
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® is a registered trademark of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

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References

References: Mantel PY, Schmidt-Weber CB. Transforming growth factor-beta: recent advances on its role in immune tolerance. Methods Mol Biol. 2011;677:303-38.

Oida T, Weiner HL. Overexpression of TGF-beta 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-beta. J Immunol. 2010 Sep 15;185(6):3529-35 (TW4-2F8, FC, WB, IP, Pubmed)

Tran DQ, Andersson J, Wang R, Ramsey H, Unutmaz D, Shevach EM. GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells. Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13445-50.

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Antigens	Area of Biology	Cell Type	Formats
LAP	Adaptive Immunity Cytokines, Chemokines, Growth Factors	NK & NKT Cells T Cells Treg Cells	Alexa Fluor 488