IKKα InstantOne ELISA Kit Performance
IKK's (IκB kinases), IKKα (IKK1) and IKKβ (IKK2), phosphorylate and trigger the degradation of the cytoplasmic NFkB inhibitors IkB (IκBa, IκBβ and IκBγ). This phosphorylation and subsequent protein degradation results in the release of NFκB proteins from its inhibitory complex and thus allows for the translocation of NFκB into the nucleus.
IKKα InstantOne ELISA Data
IKKα Analysis
Cells: HeLa cells were either unstimulated (-) or stimulated with TNF alpha (10 ng/mL for 5 min) (+).
Lysis: 1 mL of Lysis Mix buffer for 10 min with shaking.
Analysis: 20 ug/analysis.
