eFluor® Organic Dyes For IHC and ICC

eBioscience offers many organic fluorescent dyes suitable for immunofluorescence
imaging, including our own eFluor organic dyes. Each eFluor dye is named according to its
peak emission, rather than excitation, enabling users to quickly understand the emission
filter that will be needed for visualization. eFluor organic dyes are fully compatible with
eFluor Nanocrystals as well as all conventional dyes, making them an ideal addition to
your multicolor staining protocols.

eFluor® 615

Designed for fluorescence imaging; offering excellent resolution and photostability

Comparison of Anti-alpha Tubulin eFluor 615 and Anti-alpha Tubulin Alexa Fluor® 594

MCF-7 cells fixed, permeabilized and stained with Anti-Human Ki-67 FITC (1 µg/mL) and Anti-alpha Tubulin eFluor 615 (top, 342ms) or Anti-alpha Tubulin Alexa Fluor 594 (bottom, 119ms). When using eFluor 615 antibody conjugates, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor 594 filter is acceptable.

eFluor® 660

Excellent specificity and photostability; use in place of Alexa Fluor® 647

Comparison of Anti-Mouse CD4 eFluor 660 and Anti-Mouse CD4 Alexa Fluor® 647

Direct conjugate staining of frozen mouse spleen section using Anti-Mouse CD4 eFluor 660 (top, 729ms) or Anti-Mouse CD4 Alexa Fluor 647 (bottom, 1225ms). Each antibody was used at 10 µg/mL and nuclei were stained with DAPI.

Multicolor Staining with eFluor® Organic Dyes

alpha Tubulin Alexa Fluor® 488, Cytokeratin 8 eFluor 615, and DAPI

Multicolor staining of fixed, permeabilized MCF-7 cells using Anti-alpha Tubulin Alexa Fluor 488 (green), Anti-Human Cytokeratin 8 eFluor 615 (red) and DAPI (blue).

CD4 FITC and Foxp3 eFluor 615

Multicolor staining of frozen mouse spleen identifies T cells and a subpopulation of regulatory T cells using Anti-Mouse CD4 FITC (green), Anti-Mouse Foxp3 eFluor 615 (red) and DAPI (blue).