eFluor® Organic Dyes

APC-eFluor® 780 Organic Dye

Replacement for APC-Alexa Fluor® 750

APC-eFluor 780 organic dye has been developed to provide a high performance tandem fluorophore for multicolor flow cytometry. It is designed for use with flow cytometry instruments equipped with a red laser and has an emission peak of 780 nm. With similar spectral characteristics to APC-Alexa Fluor 750, APC-eFluor 780 requires no changes to your instrument configuration for use, allowing you to easily add APC-eFluor 780 to your multicolor panels.

APC-eFluor 780 is replacing APC-Alexa Fluor 750 in the eBioscience product offering.

APC-eFluor® 780 Performance Comparison to APC-Alexa Fluor® 750

Performance comparison with APC-Alexa Fluor® 750

Performance comparison with APC-Alexa Fluor® 750

Human PBMCs were stained with Anti-CD3 clone UCHT1 conjugated to APC-eFluor® 780 (left panel) or APC-Alexa Fluor® 750 (middle panel).The right panel shows the overlay of both histograms.

APC-eFluor® 780 Performance Comparison to APC-H7

Comparison with APC-H7

Comparison with APC-H7

Mouse splenocytes were stained with Anti-CD8 (clone 53-6.7) conjugated to APC-eFluor® 780 (blue-solid line) or APC-H7 (red-dotted line).

More APC-eFluor® 780 Performance Comparison to APC-Alexa Fluor® 750

CD3CD8

LEFT: Mouse splenocytes were stained with either Anti-Mouse CD3 APC-eFluor 780 (17A2) (blue solid line), Anti-Mouse CD3 APC-Alexa Fluor® 750 (17A2) (pink dotted line) or isotype control (green dotted line). Cells in the lymphocyte gate were used for analysis.

RIGHT: Mouse splenocytes were stained with either Anti-Mouse CD8 APC-eFluor 780 (53-6.7) (blue solid line), Anti-Mouse CD8 APC-Alexa Fluor® 750 (53-6.7) (pink dotted line) or isotype control (green dotted line). Cells in the lymphocyte gate were used for analysis.

B220CD44

LEFT: Mouse splenocytes were stained with either Anti-Mouse/Human CD45R (B220) APC-eFluor 780 (RA3-6B2) (blue solid line), Anti-Mouse/Human CD45R (B220) APC-Alexa Fluor® 750 (RA3-6B2) (pink dotted line) or isotype control (green dotted line). Cells in the lymphocyte gate were used for analysis.

RIGHT: Mouse splenocytes were stained with either Anti-Mouse/Human CD44 APC-eFluor 780 (IM7) (blue solid line), Anti-Mouse/Human CD44 APC-Alexa Fluor® 750 (IM7) (pink dotted line) or isotype control (green dotted line). Cells in the lymphocyte gate were used for analysis.

CD45CD45.2

LEFT: Mouse bone marrow cells were stained with either Anti-Mouse CD45 APC-eFluor 780 (30-F11) (blue solid line), Anti-Mouse CD45 APC-Alexa Fluor® 750 (30-F11) (pink dotted line) or isotype control (green dotted line). Cells in the lymphocyte gate were used for analysis.

RIGHT: Mouse splenocytes were stained with either Anti-Mouse CD45.2 APC-eFluor 780 (104) (blue solid line), Anti-Mouse CD45.2 APC-Alexa Fluor® 750 (104) (pink dotted line) or isotype control (green dotted line). Cells in the lymphocyte gate were used for analysis.

GR1CD3

LEFT: Mouse splenocytes were stained with either Anti-Mouse Gr-1 APC-eFluor 780 (RB6-8C5) (blue solid line), Anti-Mkouse Gr-1 APC-Alexa Fluor® 750 (RB6-8C5) (pink dotted line) or isotype control (green dotted line). Total cells were used for analysis.

RIGHT: Human PBMC were stained with either Anti-Human CD3 APC-eFluor® 780 (UCHT1) (blue solid line), Anti-Human CD3 APC-Alexa Fluor® 750 (UCHT1) (pink dotted line) or isotype control (green dotted line). Cells in the lymphocyte gate were used for analysis.

CD4CD8

LEFT: Human PBMC were stained with either Anti-Human CD4 APC-eFluor 780 (RPA-T4) (blue solid line), Anti-Human CD4 APC-Alexa Fluor® 750 (RPA-T4) (pink dotted line) or isotype control (green dotted line). Cells in the lymphocyte gate were used for analysis.

RIGHT: Human PBMC were stained with either Anti-Human CD8 APC-eFluor 780 (RPA-T8) (blue solid line), Anti-Human CD8 APC-Alexa Fluor® 750 (RPA-T8) (pink dotted line) or isotype control (green dotted line). Cells in the lymphocyte gate were used for analysis.

CD20CD127

LEFT: Human PBMC were stained with either Anti-Human CD20 APC-eFluor® 780 (2H7) (blue solid line), Anti-Human CD20 APC-Alexa Fluor® 750 (2H7) (pink dotted line) or isotype control (green dotted line). Cells in the lymphocyte gate were used for analysis.

RIGHT: Human PBMC were stained with either Anti-Human CD127 APC-eFluor® 780 (eBioRDR5) (blue solid line), Anti-Human CD127 APC-Alexa Fluor® 750 (eBioRDR5) (pink dotted line) or isotype control (green dotted line). Cells in the lymphocyte gate were used for analysis.