Narrow Emission Spectra Requires Minimal Compensation
The requirement for flow cytometric compensation occurs when a fluorophore emits light over a broad wavelength band that is captured by more than one detector. Therefore, fluorophores with a broader emission require greater compensation values to account for spill-over into other detectors. eFluor Nanocrystals exhibit narrow emission peaks, making compensation straightforward and less difficult than with some conventional fluorophores. For details regarding compensation and filter selection, please see the Frequently Asked Questions (FAQ) section of our website.
eFluor Nanocrystal Narrow Emission Spectra
eFluor Nanocrystals have a narrow emission spectra
eFluor Nanocrystals reagents for flow cytometry exhibit narrow emission peaks and therefore, require minimal compensation.
| Fluorochrome | Dichroic Pass Filter | Bandpass Filter | Alternative Bandpass Filters |
|---|---|---|---|
| eFluor 605NC | 595 LP | 605/40 | 605/50, 605/20, 610/20 |
| eFluor 625NC |
595 LP | 605/50 | 605/40, 625/20 |
| eFluor 650NC |
630 LP | 660/40 |
