eFluor® Nanocrystals for Flow Cytometry

Narrow Emission Spectra Requires Minimal Compensation

The requirement for flow cytometric compensation occurs when a fluorophore emits light over a broad wavelength band that is captured by more than one detector. Therefore, fluorophores with a broader emission require greater compensation values to account for spill-over into other detectors. eFluor Nanocrystals exhibit narrow emission peaks, making compensation straightforward and less difficult than with some conventional fluorophores. For details regarding compensation and filter selection, please see the Frequently Asked Questions (FAQ) section of our website.

eFluor Nanocrystal Narrow Emission Spectra

eFluor® Nanocrystals have a narrow emission spectra

eFluor Nanocrystals have a narrow emission spectra

eFluor Nanocrystals reagents for flow cytometry exhibit narrow emission peaks and therefore, require minimal compensation.

 

Recommended Filters for eFluor Nanocrystals in Flow Cytometry
Fluorochrome Dichroic Pass Filter Bandpass Filter Alternative Bandpass Filters
eFluor 605NC 595 LP 605/40 605/50, 605/20, 610/20
eFluor 625NC
595 LP 605/50 605/40, 625/20
eFluor 650NC
630 LP 660/40