eFluor® Nanocrystals in IHC and ICC
These high quantum yield nanocrystals are composed of a core particle surrounded by a unique surface coating which reduces background staining and artifacts. eFluor Nanocrystals possess intrinsic spectral properties which allow them to emit light at a distinct wavelength when excited by a single wavelength light source. Using eFluor Nanocrystals for immunofluorescence allows you to take advantage of their inherent photostability as nanocrystals are resistant to fading, even over long periods of illumination.
- Exceptionally photostable – provides better archival capabilities.
- Narrow emission spectra – provides increased multiplexing capability.
- Compatible with organic dyes – maximize the data that can be obtained from a single sample.
- Minimal background – unique outer coating reduces non-specific binding or background.
- Flexible – use for staining cultured cells, as well as frozen or paraffin-embedded tissue samples.
eFluor Nanocrystal Antibody Conjugate Representation
Nanocrystal Excitation and Emission Spectra
eFluor Nanocrystal-conjugated Antibodes for Immune Cell Phenotyping
Immune cell marker staining highlights structure/function relationship of multiple immune cell types in splenic tissue
Mouse spleen stained using Anti-Mouse CD4 eFluor 625NC (Green), Anti-Mouse CD11b eFluor 605NC (Aqua), Anti-Mouse CD11c eFluor 650NC (Red), and Anti-Mouse CD45R (B220) eFluor 525NC (Blue).Immunofluorescence visualized using a Nuance Multispectral Imaging System (Caliper Life Sciences).
Mouse CD4 eFluor 625NC and Mouse CD8a eFluor 565NC
T cell subsets are identified in frozen mouse spleen stained using Anti-Mouse CD4 eFluor 625NC (Red) and Anti-Mouse CD8a eFluor 565NC (Green).
Mouse CD11b eFluor 605NC and Human/Mouse CD45R eFluor 650NC
Myeloid cells and B cells are identified in frozen mouse spleen stained using Anti-Mouse CD11b eFluor 605NC (Green) and Anti-Human/Mouse CD45R (B220) eFluor 650NC (Red).
Mouse CD4 eFluor 605NC and Mouse/Rat Foxp3 eFluor 650NC
T cells and a subpopulation of regulatory T cells are identified in frozen mouse spleen stained using Anti-Mouse CD4 eFluor 605NC (green) and Anti-Human/Mouse Foxp3 eFluor 650NC (red).
eFluor Nanocrystals are fully compatible with organic dyes
Compatibility with traditional organic dyes allow you to maximize the data that can be obtained from a single sample by expanding your multi-color options.
Mouse CD11c eFluor 650NC, CD11b eFluor 605NC and DRAQ5™
Dendritic cells and myeloid cells are identified in frozen mouse spleen stained using Anti-Mouse CD11c eFluor 650NC (red), Anti-Mouse CD11b eFluor 605NC (blue) and nuclear counterstaining with DRAQ5 (green).
Human Cytokeratin eFluor 605 NC, alpha Tubulin eFluor 650NC and Ki-67 Alexa Fluor® 647
Multicolor staining of fixed, permeabilized MCF-7 cells stained with Anti-Human Cytokeratin eFluor 605NC (Green), Anti-alpha Tubulin eFluor 650NC (Red), and Anti-Human Ki-67 Alexa Fluor® 647 (Blue).