eFluor® Cell Proliferation Dyes
These membrane-permeable fluorescent dyes bind to cellular proteins containing primary amines; as cells divide, the dye is distributed equally between daughter cells. Cell division is therefore easily measured by flow cytometry as successive halving of the fluorescence intensity of the dye.
Cells labeled with eFluor Cell Proliferation Dyes may be fixed and permeabilized using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers, allowing further analysis of intracellular targets. Each dye is named after its emission spectra to enable ease of selection for use in multicolor flow cytometry staining panels. Cell Proliferation Dyes are also used for long term tracking of labeled cells which have been introduced in vivo by adoptive transfer.
- Visualize up to 7 generations
- Use in vitro and in vivo
- Ideal for use in combination with GFP or YFP
Cell Proliferation Dye eFluor 450 - Ideal for the Violet Laser
Cell Proliferation Dye eFluor 670 - Excited by the Red Laser
Selected publications utilizing Cell Proliferation Dye eFluor 670:
- Zhou et al. J Immunol 2011; 187, 4170-4177
- Xu et al. J Immunol 2011; 186, 6597-6606
CFSE Proliferation Dye
CFSE [5-(and 6)-Carboxyfluorescein diacetate succinimidyl ester] is an established reagent for analyzing cell proliferation. The non-fluorescent CFSE dye readily crosses intact cell membranes, where intracellular esterases cleave its acetate groups to yield the fluorescent carboxyfluorescein molecule. Cell division can be measured as successive halving of the fluorescence intensity of dye with a peak excitation of 494 nm and peak emission of 521 nm.
CyTRAK Orange™ and DRAQ5™ DNA Labeling Dyes
These are membrane-permeable anthraquinone dyes with high affinity for double stranded DNA. They are membrane-permeable, and can label live or fixed/dead cells. DRAQ5™ can be used in fluorescence microscopy applications as a nuclear counterstain for distinguishing nucleated and non-nucleated cells. CyTRAK Orange™ is ideal for fluorescent microscopy; it can be used to identify and discriminate the nucleus and cytoplasm without the need for a second dye due to its high intensity staining of the nucleus and low intensity staining of the cytoplasm.