Annexin V Apoptosis Detection Kits
Annexins are a family of calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS) to identify apoptotic cells. In healthy cells, PS is predominantly located along the cytosolic side of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution in the phospholipid bilayer and translocates to the extracellular membrane, which is detectable with fluorescently labeled Annexin V. In early stages of apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI) and 7-AAD, therefore cells which display only Annexin V staining (PI/7-AAD negative) are in early stages of apoptosis. During late-stage apoptosis, loss of cell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptake of PI and 7-AAD. Annexin V staining, paired with 7-AAD or PI is widely used to identify apoptotic stages by flow cytometry.
Features of eBioscience Annexin V Apoptosis Detection Kits
- Annexin V Apoptosis Detection Kits offer simple, reproducible options for monitoring the progression of Apoptosis using flow cytometry.
- Available in APC, FITC, PE, and our own eFluor® 450 and PerCP-eFluor® 710 formats
Annexin V Apoptosis Detection Kits Performance
Annexin V eFluor® 450
Mouse thymocytes were prepared as a single cell suspension and incubated overnight at 37°C in medium alone (left) or medium with 1 uM dexamethasone (right). Cells were harvested and stained using the Annexin V Apoptosis Detection Kit eFluor 450 and Propidium Iodide Staining Solution.
Annexin V PerCP-eFluor® 710
Mouse thymocytes were prepared as a single cell suspension and incubated overnight at 37°C in medium. Cells were harvested and stained using the Annexin V Apoptosis Detection Kit PerCP-eFluor 710 and Fixable Viability Dye eFluor® 780.
