Violet Laser Fluorophore Comparison
The eFluor solution for the violet laser line (405 nm) is one organic based dye (eFluor 450) and two nanocrystals (eFluor 605NC and eFluor 650NC). The combination of these three dyes has allowed for superior optical performance on the violet laser line with:
- High signal to noise ratios for less background.
- Minimal intra-laser compensation requirements for greater accuracy.
eFluor Violet Laser Reagents Compared to Traditional Dyes
eFluor Reagents for the Violet Laser
To illustrate the quality performance of eFluor reagents for the violet laser, staining examples for each fluorophore are shown. Human peripheral blood cells were stained with eFluor 450 Anti-Human CD45RA or the same antibody clone conjugated to Pacific Blue® (left plot). Mouse splenocytes were stained with eFluor 605NC Anti-Mouse CD8 or the same antibody clone conjugated to Pacific Orange® (middle plot) and eFluor 650NC Anti-Mouse B220 or the same antibody clone conjugated to Qdot® 655 (right plot).