eFluor Compensation and Stain Index for the Violet Laser
The emission profiles of eFluor 450, eFluor 605NC and eFluor 650NC are sufficiently narrow and well separated that the intra-laser compensation required when using all three fluorochromes simultaneously is less than 5% in any violet laser detector. The resulting benefits of reduced compensation reqirements include minimal data spread, resolution of dim and bright subpopulations and improved separation of positive and negative populations as seen by calculating the relative stain index.
Compensation Requirements of Traditional Reagents
High Intra-Laser Compensation Requirements
Human PBMC were stained with the traditional fluorophores AmCyan Anti-Human CD3, Pacific Blue® Anti-Human CD4, and Pacific Orange® Anti-Human CD8. The required compensation values for data plots are shown.
Compensation Requirements of eFluor Reagents
Minimal Intra-Laser Compensation Requirements
Human PBMC were stained with the eFluor solution of eFluor® 650NC Anti-Human CD3, eFluor 450 Anti-Human CD4, and eFluor 605NC Anti-Human CD8. The required compensation values for data plots are shown.
Relative Stain Index
eFluor - Brighter Violet Laser Reagents
From the data shown above, the relative stain index was calculated from the compensated data for each antibody used in the staining panels. The percent improvement offered by the eFluor reagents over the traditional fluorophores is shown for each specificity.