Flow Cytometry: OneComp eBeads Compensation Beads

Take the guesswork out of compensation

IL-13 staining on cells

• Broad distribution.
• Few positive events.
• Difficult to identify positive population.

IL-13 staining on OneComp eBeads

• Discrete distribution.
• 50% positive.
• Easy to identify positive population.

Advantages of Compensation Beads

• Ensure correct compensation with a guaranteed positive & negative population.
• Beads provide a bright signal for optimizing compensation.
• The antigen may be dimly expressed in sample cells.
• Compensate with the antibody used in the experiment and not a bright CD4 substitute.
• No need to search in the freezer for cells.
• Bimodal distribution can be used for single-color compensation controls in multicolor flow cytometry experiments.
• Beads can be prepared quickly on occasions when compensation has been forgotten.
• Beads are also suitable when multiple plates or large experiments are performed.
• Cells can be in limited supply making beads a great alternative.
• Best solution when conducting 6-12 color compensation.