Fluorescent Dyes for the Violet Laser

The table below lists the properties of the most commonly used fluorophores for the violet laser. To see a list of available conjugated antibodies to your fluorophore of interest, click on the fluorophore name in the table.

Violet Laser Fluorescent Dyes - 405 nm Excitation
Name Excitation (nm) Peak Emission (nm) Relative Intensity
(1 = Lowest,
5 = Highest)
Uses
eFluor® 605NC 405 605 2

Excited by the violet laser.

Brighter alternative to Pacific Orange®.

eFluor® 605NC can be used in combination with eFluor® 450, please make sure to use this conjugate for your brightest antigens / proteins of interest

eFluor® 625NC 405 625 2

Excited by the violet laser.

eFluor® 625NC can be used in combination with eFluor® 450, please make sure to use this conjugate for your brightest antigens / proteins of interest.

eFluor® 650NC 405 650 3

Excited by the violet laser.

eFluor® 650NC can be used in combination with eFluor® 450 and eFluor® 605NC, please make sure to use this conjugate for your brightest antigens / proteins of interest.

eFluor® 450 405 450 2

Excited by the violet laser.

eFluor® 450 can be used interchangeably with Pacific Blue®.

Can be used in combination with eFluor® 605NC or eFluor® 625NC, please make sure to use this conjugate for your brightest antigens / proteins of interest.

Alexa Fluor® 405

(Not available from eBioscience)

405 421 1

Extremely dim dye excited by the violet laser.

Suitable for only the brightest antigens.

Pacific Blue®

(Not available from eBioscience)

405 455 2

Excited by the violet laser.

Pacific Blue® can be used interchangeably with eFluor® 450.

Typically a dimmer fluorophore.

Please make sure to use this conjugate for your brightest antigens / proteins of interest.

Alexa Fluor® 430

(Not available from eBioscience)

405 541 1 Can be used as second dye excited by the violet laser.

Pacific Orange®

(Not available from eBioscience)

405 551 1

Dim and only suitable for densely expressed targets.

Due to its 551nm emission wavelength the Pacific Orange® dye can be used with Pacific Blue® for multicolor analysis with the violet laser.

eFluor® 605NC is a brighter alternative.