The table below lists the properties of the most commonly used fluorophores for the violet laser. To see a list of available conjugated antibodies to your fluorophore of interest, click on the fluorophore name in the table.
| Name | Excitation (nm) | Peak Emission (nm) | Relative Intensity (1 = Lowest, 5 = Highest) | Uses |
|---|---|---|---|---|
| eFluor® 605NC | 405 | 605 | 2 |
Excited by the violet laser. Brighter alternative to Pacific Orange®. eFluor® 605NC can be used in combination with eFluor® 450, please make sure to use this conjugate for your brightest antigens / proteins of interest |
| eFluor® 625NC | 405 | 625 | 2 |
Excited by the violet laser. eFluor® 625NC can be used in combination with eFluor® 450, please make sure to use this conjugate for your brightest antigens / proteins of interest. |
| eFluor® 650NC | 405 | 650 | 3 |
Excited by the violet laser. eFluor® 650NC can be used in combination with eFluor® 450 and eFluor® 605NC, please make sure to use this conjugate for your brightest antigens / proteins of interest. |
| eFluor® 450 | 405 | 450 | 2 |
Excited by the violet laser. eFluor® 450 can be used interchangeably with Pacific Blue®. Can be used in combination with eFluor® 605NC or eFluor® 625NC, please make sure to use this conjugate for your brightest antigens / proteins of interest. |
|
Alexa Fluor® 405 (Not available from eBioscience) |
405 | 421 | 1 |
Extremely dim dye excited by the violet laser. Suitable for only the brightest antigens. |
|
(Not available from eBioscience) |
405 | 455 | 2 |
Excited by the violet laser. Pacific Blue® can be used interchangeably with eFluor® 450. Typically a dimmer fluorophore. Please make sure to use this conjugate for your brightest antigens / proteins of interest. |
|
(Not available from eBioscience) |
405 | 541 | 1 | Can be used as second dye excited by the violet laser. |
|
Pacific Orange® (Not available from eBioscience) |
405 | 551 | 1 |
Dim and only suitable for densely expressed targets. Due to its 551nm emission wavelength the Pacific Orange® dye can be used with Pacific Blue® for multicolor analysis with the violet laser. eFluor® 605NC is a brighter alternative. |
