The table below lists the properties of the most commonly used fluorophores for the red laser. To see a list of available conjugated antibodies to your fluorophore of interest, click on the fluorophore name in the table.
| Name | Excitation (nm) | Peak Emission (nm) | Relative Intensity (1 = Lowest, 5 = Highest) | Uses |
|---|---|---|---|---|
| APC (Allophycocyanin) | 633 |
660 |
4 |
Detected in FL4 on dual laser instruments. Can be used interchangeably with Cy5 or Alexa Fluor® 647 |
| eFluor® 660 | 633 | 660 | 4 |
eFluor® 660 is a replacement for Alexa Fluor® 647. Detected in FL4 on dual laser instruments. Can be used interchangeably with APC. Photostable, making it a great choice for microscopy, in addition to flow cytometry. |
| Alexa Fluor® 647 | 633 | 668 | 4 |
Detected in FL4 on dual laser instruments. Can be used interchangeably with Cy5 or APC. Photostable, making it a great choice for microscopy, in addition to flow cytometry. |
| Cy5 | 633 | 670 | 3 |
Detected in FL4 on dual laser instruments (used interchangeably with APC or Alexa Fluor® 647). Good choice for intracellular labeling and microscopy. |
|
APC-Cy5.5 (Not available from eBioscience) |
633 | 690 | 3 |
Detected in appropriate channel based on filter set arrangement. Tandem dye, resonance energy transfer from APC molecule to Cy5.5. |
| Alexa Fluor® 700 | 633 | 723 | 2 |
Although not optimally excited by the He-Ne laser, it does produce a bright signal that can be used as a third dye off this laser. Please note that emission wavelength requires a distinct set of filters. Most instruments will require a 685 LP mirror and 710/20 filter. |
| APC-eFluor® 780 |
633 |
780 |
2 |
Appropriate channel for detection based on filter set arrangement of the instrument. More photostable than APC-Cy7. Tandem dye, resonance energy transfer from APC molecule to eFluor® 780. APC-Cy7, APC-Alexa Fluor® 750 and APC-eFluor® 780 may be used interchangeably. |
|
(Not available from eBioscience) |
633 | 775 | 2 |
Appropriate channel for detection based on filter set arrangement of the instrument. More photostable than APC-Cy7. Tandem dye, resonance energy transfer from APC molecule to Alexa Fluor® 750. APC-Cy7, APC-Alexa Fluor® 750 and APC-eFluor® 780 may be used interchangeably. |
