The table below lists the properties of the most commonly used fluorophores for the green laser. To see a list of available conjugated antibodies to your fluorophore of interest, click on the fluorophore name in the table.
| Name | Excitation (nm) | Peak Emission (nm) | Relative Intensity (1 = Lowest, 5 = Highest) | Uses |
|---|---|---|---|---|
| 532 | 561 | 3 |
Detected in channel with appropriate filter set arrangement for the instrument. Photostable and pH independent. An excellent choice for fluorescence microscopy. |
|
| R-PE (R-Phycoerythrin) | 532 - 561 |
578 | 5 |
Detected in FL2 channel on most instruments. High quantum yield (ideal for detection of low density antigens). Subject to photobleaching. |
|
PE-Texas Red (Not available from eBioscience) |
532 - 561 |
615 | 3 | Detected in FL3 on single laser instruments. |
| PE-Cy5 | 532 - 561 |
667 | 4 |
Detected in FL3 or FL4 on most instruments. Tandem dye, resonance energy transfer from PE molecule to the cyanine dye Cy5. When used with APC on dual laser machines, needs a cytometer capable of interlaser compensation. See PE-Cy5.5 for dual laser instruments. |
| 532 - 561 |
695 | 3 to 4 |
Detected in FL3 or FL4 on most instruments. Tandem dye, resonance energy transfer from PE molecule to cyanine dye Cy5.5. When used with APC on dual laser machines, needs a cytometer capable of interlaser compensation. Better choice for dual laser instruments than PE-Cy5. |
|
| PE-Cy7 | 532 - 561 |
785 | 3 |
Detected in channel with appropriate filter set arrangement for the instrument. Tandem dye, resonance energy transfer from PE molecule to cyanine dye Cy7. Minimal cross-beam compensation required when used simultaneously. |
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