The table below lists the properties of the most commonly used fluorophores for the blue laser. To see a list of available conjugated antibodies to your fluorophore of interest, click on the fluorophore name in the table.
| Name | Excitation (nm) | Peak Emission (nm) | Relative Intensity (1 = Lowest, 5 = Highest) | Uses |
|---|---|---|---|---|
| FITC (Fluorescein Isothiocyanate) | 488 | 518 | 3 |
Detected in FL1 channel on most instruments. Also a good choice for fluorescence microscopy. Useful in fluorescent microscopy applications. |
| Alexa Fluor® 488 | 488 | 519 | 3 |
Detected in FL1 channel on most instruments. Photostable and pH dependent. An excellent choice for fluorescence microscopy. |
| R-PE (R-Phycoerythrin) | 488 | 578 | 5 |
Detected in FL2 channel on most instruments. High quantum yield (ideal for detection of low density antigens). Subject to photobleaching. |
|
PE-Texas Red (Not available from eBioscience) |
488 | 615 | 3 | Detected in FL3 on single laser instruments. |
|
PE-Alexa Fluor® 610 (Not available from eBioscience) |
488 | 628 | 3 | This alternative to PE-Texas Red has less overlap with PE due to its 628nm emission wavelength and is less subject to Fc mediated binding than PE-Cy5. |
| PE-Cy5 | 488 | 667 | 4 |
Detected in FL3 or FL4 on most instruments. Tandem dye, resonance energy transfer from PE molecule to the cyanine dye Cy5. When used with APC on dual laser machines, needs a cytometer capable of interlaser compensation. See PE-Cy5.5 for dual laser instruments. |
|
PE-Cy5.5 (Coming Soon!) |
488 | 695 | 3 to 4 |
Detected in FL3 or FL4 on most instruments. Tandem dye, resonance energy transfer from PE molecule to cyanine dye Cy5.5. When used with APC on dual laser machines, needs a cytometer capable of interlaser compensation. Better choice for dual laser instruments than PE-Cy5. |
| PerCP-Cy5.5 | 488 | 695 | 3 |
Detected in FL3 or FL4 on most instruments. Tandem dye, resonance energy transfer from PerCP molecule to cyanine dye Cy5.5. When used with APC on dual laser machines, needs a cytometer capable of inter-laser compensation. Better choice for dual laser instruments than PE-tandems as a result of its large stokes shift. |
| PerCP-eFluor® 710 | 488 | 710 | 4 |
Detected in FL3 on most instruments. Tandem dye, resonance energy transfer from PerCP molecule to eFluor® 710. When used with APC on dual laser machines, needs a cytometer capable of inter-laser compensation. Better choice for dual laser instruments than PE-tandems as a result of its large stokes shift. 2-3 fold brighter than PerCP-Cy5.5. |
| PE-Cy7 | 488 | 785 | 3 |
Detected in channel with appropriate filter set arrangement for the instrument. Tandem dye, resonance energy transfer from PE molecule to cyanine dye Cy7. Minimal cross-beam compensation required when used simultaneously. |
| 7-AAD | 488 | 647 | -- |
Detected in FL3 channel on most instruments. Binds selectively to GC regions of DNA. Used as a viability dye to discriminate between viable and non-viable cells. |
| Propidium Iodide (PI) | 488 | 617 |
-- |
Detected in FL2 and FL3 channels on most instruments. A DNA intercalating dye. Used as a viability dye to discriminate between viable and nonviable cells. |
