IKKa (Total/Phospho) InstantOne™ ELISA

Also known as: Inhibitor of nuclear factor kappa-B kinase subunit alpha

RUO: For Research Use Only. Not for use in diagnostic procedures.

Attention: The Total/Phospho InstantOne ELISAs contain reagents for a total of 96 wells (24 wells total/72 wells phospho). Please contact Technical Service for a lot specific COA.

SKU# 85-86073

Cat. No.	Size
85-86073-11	96 tests
Please inquire about custom configurations or bulk packaging.

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Serum-starved HeLa cells were unstimulated (-) or stimulated with TNF alpha (10 ng/mL for 5 min)...View More

Description

Description: The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human IKKa (Ser176/180) in cell lysates. Detection of total and phosphorylated mouse and rat IKKa (Ser176/180) is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

The IkB kinases, IKKa (IKK1) and IKKb (IKK2), phosphorylate and trigger degradation of the cytoplasmic NFkB inhibitors IkB (IkBa, IkBb, and IkBg). These events result in the release of NFkB proteins from its inhibitory complex in the cytosol and enables NFkB to translocate into the nucleus to regulate transcription.

Details

Reactivity	Human
Suitable Sample Types	Tested on cell culture samples. Expected to work on many other sample types, but have not been tested at this time.
Components	The kit includes sufficient reagents to perform 24 wells of the total and 72 wells of the phospho-specific detection for a total of 96 wells. User Manual InstantOne™ Assay Plate (Part No. IOAP96) - 96 well (sealed) Cell Lysis Buffer (5X) (Part No. IOCLB1) - 8 mL Enhancer Solution (Part No. IOES1) - 0.5 mL Wash Buffer (10X) (Part No. IOWB1) - 8 mL IKKa (Total) Capture Antibody Reagent (Part No. IOIKKCAR1M) - 1 x 0.75 mL phospho-IKKa (Ser176/180) Capture Antibody Reagent (Part No. IOPIKKCAR1M) - 3 x 0.75 mL IKKa (Total) Detection Antibody Reagent (Part No. IOIKKDAR1M) - 1 x 0.75 mL phospho-IKKa (Ser176/180) Detection Antibody Reagent (Part No. IOPIKKDAR1M) - 3 x 0.75 mL Positive Control Cell Lysate (lyophilized) (Part No. IOIKKPC1) - 1 ea Detection Reagent (TMB Substrate) (Part No. IODR1) - 10 mL Stop Solution (Part No. IOSS1) - 10 mL Plate Seal - 1 ea
Reported Applications	ELISA

Documentation

TDS Link	Download TDS
User Manual	Download User Manual

Related Products

Cat. No.	Name	Application	Reg.
IOCLB	InstantOne ELISA Cell Lysis Buffer (5X)	ELISA	RUO
IOES	InstantOne ELISA Enhancer Solution	ELISA	RUO
IOWB	InstantOne ELISA Wash Buffer(10X)	ELISA	RUO
85-86071	IKKa (Total) InstantOne™ ELISA	ELISA	RUO
85-86072	phospho-IKKa (Ser176/180) InstantOne™ ELISA	ELISA	RUO
85-86084	NFkB Pathway Activation InstantOne™ ELISA	ELISA	RUO

References

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Area of Biology
Cell Signaling