Description: The 2F3.2 monoclonal antibody reacts with human zeta-chain associated protein 70 (ZAP-70), a cytoplasmic tyrosine kinase expressed in T cells, natural killer cells, as well as in developing and some mature B cell subsets. Upon T cell activation, ZAP-70 is recruited to the T cell receptor (TCR) and subsequently phosphorylates signaling molecules such as LAT and SLP-76. In addition to its role in TCR signaling, ZAP-70 has been implicated in thymocyte development.
ZAP-70 is also a surrogate biomarker for determining chronic lymphocytic leukemia (CLL) prognosis. Expression of this kinase is used to indicate the somatic mutational status of the immunoglobulin variable heavy chain gene (IgVH). Genomic studies revealed that ZAP-70 gene expression correlates with an unmutated IgVH gene.