Human VEGF-R3/FLT-4 Platinum ELISA

Description: The human VEGF-R3/FLT-4 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human VEGF-R3/FLT-4. The human VEGF-R3/FLT-4 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

The main function of the lymphatic system is to return plasma back to the circulating blood. Plasma fluid, macromolecules and cells, such as leucocytes and activated antigen-presenting cells, enter the blind-ended lymphatic vessels. Lymph is then transported toward collecting lymphatic vessels and is returned to the blood circulation.

Scientific studies over the past years have revealed a signal-transduction system for lymphatic endothelial cell growth, migration and survival. This system is formed by VEGF-C and VEGF-D and their shared receptor VEGFR-3/FLT-4. Homozygous deletion of VEGFR-3 in mice leads to defects in blood-vessel remodelling and embryonic death at mid-gestation, indicating an early vascular function for the receptor. Furthermore mouse studies suggest that upregulation and activation of VEGFR-3 have functional roles in sprouting angiogenesis and that VEGFR-3 is an important effector of the vascular phenotype resulting from Notch inhibition.

In lymphoma patients, high expression of VEGFR-3 can been found in lymph endothelial cells isolated from the tumour tissue.

References: Olsson,A.K.; Dimberg,A.; Kreuger,J.; Claesson-Welsh,L.. VEGF receptor signalling - in control of vascular function. Nat.Rev.Mol Cell Biol 2006;7:359-371. (Link)

Tammela,T.; Petrova,T.V.; Alitalo,K.. Molecular lymphangiogenesis: new players. Trends Cell Biol 2005;15:434-441. (Link)

Alitalo,K.; Tammela,T.; Petrova,T.V.. Lymphangiogenesis in development and human disease. Nature 2005;438:946-953. (Link)

Oliver,G.. Lymphatic vasculature development. Nat.Rev.Immunol. 2004;4:35-45. (Link)

Norgall,S.; Papoutsi,M.; Rossler,J.; Schweigerer,L.; Wilting,J.; Weich,H.A.. Elevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomas. BMC Cancer 2007;7:105(Link)

Ferrara,N.; Kerbel,R.S.. Angiogenesis as a therapeutic target. Nature 2005;438:967-974. (Link)

Tammela,T.; Zarkada,G.; Wallgard,E.; Murtomaki,A.; Suchting,S.; Wirzenius,M.; Waltari,M.; Hellstrom,M.; Schomber,T.; Peltonen,R.; Freitas,C.; Duarte,A.; Isoniemi,H.; Laakkonen,P.; Christofori,G.; Yla-Herttuala,S.; Shibuya,M.; Pytowski,B.; Eichmann,A.; Betsholtz,C.; Alitalo,K.. Blocking VEGFR-3 suppresses angiogenic sprouting and vascular network formation. Nature 2008;454:656-660. (Link)