Human TNF-beta FlowCytomix Simplex

Description: This FlowCytomix Simplex Kit is designed for the measurement of Human TNF-beta in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit (cat. BMS8420FF), this kit can be used to detect TNF-beta alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.

This kit contains bead population B10.

TNF-beta is produced by activated lymphocytes, whereas TNF-alpha is mostly produced by activated macrophages. Soluble TNF-beta is a T-cell derived glycoprotein of 25 kD encoded by a gene within the MHC. The molecule consists of a 17.5 to 18 kD polypeptide core and 7kD of N-linked carbohydrate. TNF-beta is 28% structurally homologous with the macrophage produced non-glycoprotein TNF-alpha.

TNF-beta is induced in an antigen-specific MHC restricted fashion from class I and class II restricted T cells. Viral infection is also associated with TNF-beta production by lymphoid cells. TNF-beta has several effects on target cells including killing, growth stimulation, induction of adhesion molecule (ICAM-1) expression, and induction of differentiation. The mechanisms of TNF-beta effects involve receptor binding and internalization and several sequelae including changes in prostaglandins and chromosome integrity. TNF-beta participates in tumor immunity, and it has been reported to inhibit carcinogenesis as well as growth of some tumors in vivo.

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