Human TNF-beta Instant ELISA

Description: The human TNF-beta ELISA is for in vitro diagnostic use. Not for use in therapeutic procedures.

The human TNF-beta ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human TNF-beta. The human TNF-beta ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Tumor Necrosis Factor (TNF) was originally discovered as a serum protein with necrotizing effects on certain transplantable mouse tumors in vivo and cytotoxic effects against some transformed cells in vitro. The TNF family consists of two proteins designated TNF-alpha, also called cachectin, and TNF-beta, also called lymphotoxin. Soluble TNF-beta is a T-cell derived glycoprotein of 25 kD mainly produced by activated lymphocytes. TNF-beta is 28 % structurally homologous with the macrophage produced non-glycoprotein TNF-alpha.

TNF-beta is induced in an antigen-specific MHC restricted fashion from class I and class II restricted T cells. Viral infection is also associated with TNF-beta production by lymphoid cells. TNF-beta has several effects on target cells including killing, growth stimulation, induction of adhesion molecule (ICAM-1) expression, and induction of differentiation. The mechanisms of TNF-beta effects involve receptor binding and internalization and several sequelae including changes in prostaglandins and chromosome integrity. TNF-beta participates in tumor immunity, and it has been reported to inhibit carcinogenesis as well as growth of some tumors in vivo.

The assay detects recombinant, unglycosylated lymphotoxin with the same sensitivity as the natural, glycosylated protein, shows good correlation with the standard cytotoxicity bioassay, and is specific for biologically active TNF-beta without TNF-alpha cross reactivity.

Citations: Scholz C.,L St„rck,G Willimsky,T Blankenstein ,B D”rken and P T Daniel. Adenoviral transduction of tumor cells induces apoptosis in co-cultured T lymphocytes. Gene Therapy 2002;9:1438-1446. (Link)

Beatty,P.R.; Krams,S.M.; Martinez,O.M.. Involvement of IL-10 in the autonomous growth of EBV-transformed B cell lines. The Journal of Immunology 1997;158:4045-4051. (Link)

Verhasselt,Valerie; Vanden Berghe,Wim; Vanderheyde,Nathalie; Willems,Fabienne; Haegeman,Guy; Goldman,Michel. N-Acetyl-L-Cysteine Inhibits Primary Human T Cell Responses at the Dendritic Cell Level: Association with NF-{kappa}B Inhibition. The Journal of Immunology 1999;162:2569-2574. (Link)

Noraz,Nelly; Lathey,Janet L.; Spector,Stephen A.. Human Cytomegalovirus-Associated Immunosuppression Is Mediated Through Interferon-alpha. Blood 1997;89:2443-2452. (Link)

Laske,C.; Oschmann,P.; Tofighi,J.; Kuehne,S.B.; Diehl,H.; Bregenzer,T.; Kraus,J.; Bauer,R.; Chatzimanolis,N.; Kern,A.; Traupe,H.; Kaps,M.. Induction of sTNF-R1 and sTNF-R2 by interferon beta-1b in correlation with clinical and MRI activity. Acta Neurologica Scandinavica 2001;103:105-113. (Link)

Steiner,G.; Tohidast-Akrad,M.; Witzmann,G.; Vesely,M.; Studnicka-Benke,A.; Gal,A.; Kunaver,M.; Zenz,P.; Smolen,J.S.. Cytokine production by synovial T cells in rheumatoid arthritis. Rheumatology 1999;38:202-213. (Link)

Partsch,Gerald; Wagner,Ernst; Leeb,Burkhard F.; Broll,Hans; Dunky,Attila; Smolen,Josef S.. T cell derived cytokines in psoriatic arthritis synovial fluids. Annals of the Rheumatic Diseases 1998;57:691-693. (Link)

References: Li,C.B.; Gray,P.W.; Lin,P.F.; McGrath,K.M.; Ruddle,F.H.; Ruddle,N.H.. Cloning and expression of murine lymphotoxin cDNA. J.Immunol. 1987;138:4496-4501. (Link)

Bengtsson,A.K.; Ryan,E.J.. Immune function of the decoy receptor osteoprotegerin. Crit Rev.Immunol. 2002;22:201-215. (Link)

Spies,T.; Morton,C.C.; Nedospasov,S.A.; Fiers,W.; Pious,D.; Strominger,J.L.. Genes for the tumor necrosis factors alpha and beta are linked to the human major histocompatibility complex. Proc.Natl.Acad.Sci.U.S.A 1986;83:8699-8702. (Link)

Scheurich,P.; Thoma,B.; Ucer,U.; Pfizenmaier,K.. Immunoregulatory activity of recombinant human tumor necrosis factor (TNF)-alpha: induction of TNF receptors on human T cells and TNF-alpha-mediated enhancement of T cell responses. J.Immunol. 1987;138:1786-1790. (Link)

Gray,P.W.; Aggarwal,B.B.; Benton,C.V.; Bringman,T.S.; Henzel,W.J.; Jarrett,J.A.; Leung,D.W.; Moffat,B.; Ng,P.; Svedersky,L.P.; .. Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature 1984;312:721-724. (Link)

Vilcek,J.; Palombella,V.J.; Henriksen-DeStefano,D.; Swenson,C.; Feinman,R.; Hirai,M.; Tsujimoto,M.. Fibroblast growth enhancing activity of tumor necrosis factor and its relationship to other polypeptide growth factors. J.Exp.Med. 1986;163:632-643. (Link)

Adolf,G.R.; Apfler,I.. A monoclonal antibody-based enzyme immunoassay for quantitation of human tumor necrosis factor binding protein I, a soluble fragment of the 60 kDa TNF receptor, in biological fluids. J.Immunol.Methods 1991;143:127-136. (Link)

Paul,N.L.; Ruddle,N.H.. Lymphotoxin. Annu.Rev.Immunol. 1988;6:407-438. (Link)

Broudy,V.C.; Harlan,J.M.; Adamson,J.W.. Disparate effects of tumor necrosis factor-alpha/cachectin and tumor necrosis factor-beta/lymphotoxin on hematopoietic growth factor production and neutrophil adhesion molecule expression by cultured human endothelial cells. J.Immunol. 1987;138:4298-4302. (Link)

Adolf,G.R.; Lamche,H.R.. Highly sensitive enzyme immunoassay for human lymphotoxin (tumor necrosis factor beta) in serum. J.Immunol.Methods 1990;130:177-185. (Link)

Locksley,R.M.; Heinzel,F.P.; Shepard,H.M.; Agosti,J.; Eessalu,T.E.; Aggarwal,B.B.; Harlan,J.M.. Tumor necrosis factors alpha and beta differ in their capacities to generate interleukin 1 release from human endothelial cells. J.Immunol. 1987;139:1891-1895. (Link)

Exley,A.R.; Cohen,J.. Optimal collection of blood samples for the measurement of tumor necrosis factor alpha. Cytokine 1990;2:353-356. (Link)

Wong,G.H.; Goeddel,D.V.. Tumour necrosis factors alpha and beta inhibit virus replication and synergize with interferons. Nature 1986;323:819-822. (Link)

Himmler,A.; Maurer-Fogy,I.; Kronke,M.; Scheurich,P.; Pfizenmaier,K.; Lantz,M.; Olsson,I.; Hauptmann,R.; Stratowa,C.; Adolf,G.R.. Molecular cloning and expression of human and rat tumor necrosis factor receptor chain (p60) and its soluble derivative, tumor necrosis factor-binding protein. DNA Cell Biol. 1990;9:705-715. (Link)

Clark,I.A.; Gray,K.M.; Rockett,E.J.; Cowden,W.B.; Rockett,K.A.; Ferrante,A.; Aggarwal,B.B.. Increased lymphotoxin in human malarial serum, and the ability of this cytokine to increase plasma interleukin-6 and cause hypoglycaemia in mice: implications for malarial pathology. Trans.R.Soc.Trop.Med.Hyg. 1992;86:602-607. (Link)

Shalaby,M.R.; Aggarwal,B.B.; Rinderknecht,E.; Svedersky,L.P.; Finkle,B.S.; Palladino,M.A.,Jr.. Activation of human polymorphonuclear neutrophil functions by interferon-gamma and tumor necrosis factors. J.Immunol. 1985;135:2069-2073. (Link)

Kramer,S.M.; Carver,M.E.. Serum-free in vitro bioassay for the detection of tumor necrosis factor. J.Immunol.Methods 1986;93:201-206. (Link)

Ishibashi,K.; Ishitsuka,K.; Chuman,Y.; Otsuka,M.; Kuwazuru,Y.; Iwahashi,M.; Utsunomiya,A.; Hanada,S.; Sakurami,T.; Arima,T.. Tumor necrosis factor-beta in the serum of adult T-cell leukemia with hypercalcemia. Blood 1991;77:2451-2455. (Link)

Bertolini,D.R.; Nedwin,G.E.; Bringman,T.S.; Smith,D.D.; Mundy,G.R.. Stimulation of bone resorption and inhibition of bone formation in vitro by human tumour necrosis factors. Nature 1986;319:516-518. (Link)